TY - JOUR
T1 - Optimization of biopharmaceutical downstream processes supported by mechanistic models and artificial neural networks
AU - Pirrung, Silvia M.
AU - van der Wielen, Luuk A.M.
AU - van Beckhoven, Ruud F.W.C.
AU - van de Sandt, Emile J A X
AU - Eppink, Michel H M
AU - Ottens, Marcel
PY - 2017/5/1
Y1 - 2017/5/1
N2 - Downstream process development is a major area of importance within the field of bioengineering. During the design of such a downstream process, important decisions have to be made regarding the type of unit operations as well as their sequence and their operating conditions. Current computational approaches addressing these issues either show a high level of simplification or struggle with computational speed. Therefore, this article presents a new approach that combines detailed mechanistic models and speed-enhancing artificial neural networks. This approach was able to simultaneously optimize a process with three different chromatographic columns toward yield with a minimum purity of 99.9%. The addition of artificial neural networks greatly accelerated this optimization. Due to high computational speed, the approach is easily extendable to include more unit operations. Therefore, it can be of great help in the acceleration of downstream process development.
AB - Downstream process development is a major area of importance within the field of bioengineering. During the design of such a downstream process, important decisions have to be made regarding the type of unit operations as well as their sequence and their operating conditions. Current computational approaches addressing these issues either show a high level of simplification or struggle with computational speed. Therefore, this article presents a new approach that combines detailed mechanistic models and speed-enhancing artificial neural networks. This approach was able to simultaneously optimize a process with three different chromatographic columns toward yield with a minimum purity of 99.9%. The addition of artificial neural networks greatly accelerated this optimization. Due to high computational speed, the approach is easily extendable to include more unit operations. Therefore, it can be of great help in the acceleration of downstream process development.
KW - chromatography
KW - downstream processing
KW - model-based process development approach
KW - purification process synthesis
UR - http://www.scopus.com/inward/record.url?scp=85011634332&partnerID=8YFLogxK
U2 - 10.1002/btpr.2435
DO - 10.1002/btpr.2435
M3 - Article
AN - SCOPUS:85011634332
SN - 8756-7938
VL - 33
SP - 696
EP - 707
JO - Biotechnology Progress
JF - Biotechnology Progress
IS - 3
ER -