Optimizing mixing in micro-bioreactors used for high throughput screening of suitable biocatalysts in industrial fermentation

Xiaonan Li; Marcel Ottens; Gijs W.K. Van Dedem; Luuk A.M. Van Der Wielen; Michiel Van Leeuwen; Walter Van Gulik; Josef J. Heijnen; EE Krommenhoek; J. G.E. Gardeniers; A. Van Den Berg

Optimizing mixing in micro-bioreactors used for high throughput screening of suitable biocatalysts in industrial fermentation

Xiaonan Li, Marcel Ottens, Gijs W.K. Van Dedem, Luuk A.M. Van Der Wielen, Michiel Van Leeuwen, Walter Van Gulik, Josef J. Heijnen, EE Krommenhoek, J. G.E. Gardeniers, A. Van Den Berg

BT/Bioprocess Engineering

Research output: Chapter in Book/Conference proceedings/Edited volume › Conference contribution › Scientific › peer-review

Abstract

There is a growing interest in developing minute laboratories on a 'chip' (Lab-on-a-Chip) and micro-(bio)chemical reactors as clearly shown by the research efforts of many industrial companies and research institutes in recent years. The development of a new technological platform for fed batch fermentations in very small volume (10-300 μl) reactors in an array format will be described, which in the end will allow to improve production properties of micro organisms by rapid screening under industrially relevant conditions. One of the objectives (besides developing sensing technology) is to design and test methods to analyze and control mixing. This presentation addresses several mixing methods that can be used in micro-bioreactors. The micro-bioreactor is operated under extreme conditions like high biomass and product concentrations, high oxygen requirement and long residence time (3-4 days). With the help of Computational Fluid Dynamics simulations, the influence of the mixing method on the fermentation efficiency in the microreactor is described. These results will be supported by real-life experimental mixing data.

Original language	English
Title of host publication	05AIChE: 2005 AIChE Annual Meeting and Fall Showcase, Conference Proceedings
Pages	8696
Number of pages	1
Publication status	Published - 2005
Event	05AIChE: 2005 AIChE Annual Meeting and Fall Showcase - Cincinnati, OH, United States Duration: 30 Oct 2005 → 4 Nov 2005

Conference

Conference	05AIChE: 2005 AIChE Annual Meeting and Fall Showcase
Country/Territory	United States
City	Cincinnati, OH
Period	30/10/05 → 4/11/05

Cite this

Li, X., Ottens, M., Van Dedem, G. W. K., Van Der Wielen, L. A. M., Van Leeuwen, M., Van Gulik, W., Heijnen, J. J., Krommenhoek, EE., Gardeniers, J. G. E., & Van Den Berg, A. (2005). Optimizing mixing in micro-bioreactors used for high throughput screening of suitable biocatalysts in industrial fermentation. In 05AIChE: 2005 AIChE Annual Meeting and Fall Showcase, Conference Proceedings (pp. 8696)

@inproceedings{a99621853a2048059942e31b3daa271b,

title = "Optimizing mixing in micro-bioreactors used for high throughput screening of suitable biocatalysts in industrial fermentation",

abstract = "There is a growing interest in developing minute laboratories on a 'chip' (Lab-on-a-Chip) and micro-(bio)chemical reactors as clearly shown by the research efforts of many industrial companies and research institutes in recent years. The development of a new technological platform for fed batch fermentations in very small volume (10-300 μl) reactors in an array format will be described, which in the end will allow to improve production properties of micro organisms by rapid screening under industrially relevant conditions. One of the objectives (besides developing sensing technology) is to design and test methods to analyze and control mixing. This presentation addresses several mixing methods that can be used in micro-bioreactors. The micro-bioreactor is operated under extreme conditions like high biomass and product concentrations, high oxygen requirement and long residence time (3-4 days). With the help of Computational Fluid Dynamics simulations, the influence of the mixing method on the fermentation efficiency in the microreactor is described. These results will be supported by real-life experimental mixing data.",

author = "Xiaonan Li and Marcel Ottens and {Van Dedem}, {Gijs W.K.} and {Van Der Wielen}, {Luuk A.M.} and {Van Leeuwen}, Michiel and {Van Gulik}, Walter and Heijnen, {Josef J.} and EE Krommenhoek and Gardeniers, {J. G.E.} and {Van Den Berg}, A.",

year = "2005",

language = "English",

pages = "8696",

booktitle = "05AIChE: 2005 AIChE Annual Meeting and Fall Showcase, Conference Proceedings",

note = "05AIChE: 2005 AIChE Annual Meeting and Fall Showcase ; Conference date: 30-10-2005 Through 04-11-2005",

}

Li, X, Ottens, M, Van Dedem, GWK, Van Der Wielen, LAM, Van Leeuwen, M, Van Gulik, W, Heijnen, JJ, Krommenhoek, EE, Gardeniers, JGE & Van Den Berg, A 2005, Optimizing mixing in micro-bioreactors used for high throughput screening of suitable biocatalysts in industrial fermentation. in 05AIChE: 2005 AIChE Annual Meeting and Fall Showcase, Conference Proceedings. pp. 8696, 05AIChE: 2005 AIChE Annual Meeting and Fall Showcase, Cincinnati, OH, United States, 30/10/05.

Optimizing mixing in micro-bioreactors used for high throughput screening of suitable biocatalysts in industrial fermentation. / Li, Xiaonan; Ottens, Marcel; Van Dedem, Gijs W.K. et al.
05AIChE: 2005 AIChE Annual Meeting and Fall Showcase, Conference Proceedings. 2005. p. 8696.

Research output: Chapter in Book/Conference proceedings/Edited volume › Conference contribution › Scientific › peer-review

TY - GEN

T1 - Optimizing mixing in micro-bioreactors used for high throughput screening of suitable biocatalysts in industrial fermentation

AU - Li, Xiaonan

AU - Ottens, Marcel

AU - Van Dedem, Gijs W.K.

AU - Van Der Wielen, Luuk A.M.

AU - Van Leeuwen, Michiel

AU - Van Gulik, Walter

AU - Heijnen, Josef J.

AU - Krommenhoek, EE

AU - Gardeniers, J. G.E.

AU - Van Den Berg, A.

PY - 2005

Y1 - 2005

N2 - There is a growing interest in developing minute laboratories on a 'chip' (Lab-on-a-Chip) and micro-(bio)chemical reactors as clearly shown by the research efforts of many industrial companies and research institutes in recent years. The development of a new technological platform for fed batch fermentations in very small volume (10-300 μl) reactors in an array format will be described, which in the end will allow to improve production properties of micro organisms by rapid screening under industrially relevant conditions. One of the objectives (besides developing sensing technology) is to design and test methods to analyze and control mixing. This presentation addresses several mixing methods that can be used in micro-bioreactors. The micro-bioreactor is operated under extreme conditions like high biomass and product concentrations, high oxygen requirement and long residence time (3-4 days). With the help of Computational Fluid Dynamics simulations, the influence of the mixing method on the fermentation efficiency in the microreactor is described. These results will be supported by real-life experimental mixing data.

AB - There is a growing interest in developing minute laboratories on a 'chip' (Lab-on-a-Chip) and micro-(bio)chemical reactors as clearly shown by the research efforts of many industrial companies and research institutes in recent years. The development of a new technological platform for fed batch fermentations in very small volume (10-300 μl) reactors in an array format will be described, which in the end will allow to improve production properties of micro organisms by rapid screening under industrially relevant conditions. One of the objectives (besides developing sensing technology) is to design and test methods to analyze and control mixing. This presentation addresses several mixing methods that can be used in micro-bioreactors. The micro-bioreactor is operated under extreme conditions like high biomass and product concentrations, high oxygen requirement and long residence time (3-4 days). With the help of Computational Fluid Dynamics simulations, the influence of the mixing method on the fermentation efficiency in the microreactor is described. These results will be supported by real-life experimental mixing data.

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M3 - Conference contribution

AN - SCOPUS:33645651906

SP - 8696

BT - 05AIChE: 2005 AIChE Annual Meeting and Fall Showcase, Conference Proceedings

T2 - 05AIChE: 2005 AIChE Annual Meeting and Fall Showcase

Y2 - 30 October 2005 through 4 November 2005

ER -

Optimizing mixing in micro-bioreactors used for high throughput screening of suitable biocatalysts in industrial fermentation

Abstract

Conference

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