Optogenetics and Light-Sheet Microscopy

Laura Maddalena, Paolo Pozzi, Nicolò G. Ceffa, Bas van der Hoeven, Elizabeth C. Carroll*

*Corresponding author for this work

Research output: Chapter in Book/Conference proceedings/Edited volumeChapterScientific

18 Downloads (Pure)

Abstract

Light-sheet microscopy is a powerful method for imaging small translucent samples in vivo, owing to its unique combination of fast imaging speeds, large field of view, and low phototoxicity. This chapter briefly reviews state-of-the-art technology for variations of light-sheet microscopy. We review recent examples of optogenetics in combination with light-sheet microscopy and discuss some current bottlenecks and horizons of light sheet in all-optical physiology. We describe how 3-dimensional optogenetics can be added to an home-built light-sheet microscope, including technical notes about choices in microscope configuration to consider depending on the time and length scales of interest.

Original languageEnglish
Title of host publicationNeuromethods
EditorsEirini Papagiakoumou
PublisherHumana Press Inc.
Pages231-261
Volume191
ISBN (Electronic)978-1-0716-2764-8
DOIs
Publication statusPublished - 2023

Publication series

NameNeuromethods
Volume191
ISSN (Print)0893-2336
ISSN (Electronic)1940-6045

Keywords

  • Adaptive optics
  • Computer-generated holography
  • Light-sheet microscopy
  • Optogenetics
  • Zebrafish

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