@inbook{f119aa6cc6794de788b4042828441cdb,
title = "Optogenetics and Light-Sheet Microscopy",
abstract = "Light-sheet microscopy is a powerful method for imaging small translucent samples in vivo, owing to its unique combination of fast imaging speeds, large field of view, and low phototoxicity. This chapter briefly reviews state-of-the-art technology for variations of light-sheet microscopy. We review recent examples of optogenetics in combination with light-sheet microscopy and discuss some current bottlenecks and horizons of light sheet in all-optical physiology. We describe how 3-dimensional optogenetics can be added to an home-built light-sheet microscope, including technical notes about choices in microscope configuration to consider depending on the time and length scales of interest.",
keywords = "Adaptive optics, Computer-generated holography, Light-sheet microscopy, Optogenetics, Zebrafish",
author = "Laura Maddalena and Paolo Pozzi and Ceffa, {Nicol{\`o} G.} and Hoeven, {Bas van der} and Carroll, {Elizabeth C.}",
year = "2023",
doi = "10.1007/978-1-0716-2764-8_8",
language = "English",
volume = "191",
series = "Neuromethods",
publisher = "Humana Press Inc.",
pages = "231--261",
editor = "{ Papagiakoumou }, Eirini",
booktitle = "Neuromethods",
}