Pathway engineering strategies for improved product yield in yeast-based industrial ethanol production

Aafke C.A. van Aalst, Sophie C. de Valk, Walter M. van Gulik, Mickel L.A. Jansen, Jack T. Pronk, Robert Mans*

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

1 Citation (Scopus)
33 Downloads (Pure)

Abstract

Product yield on carbohydrate feedstocks is a key performance indicator for industrial ethanol production with the yeast Saccharomyces cerevisiae. This paper reviews pathway engineering strategies for improving ethanol yield on glucose and/or sucrose in anaerobic cultures of this yeast by altering the ratio of ethanol production, yeast growth and glycerol formation. Particular attention is paid to strategies aimed at altering energy coupling of alcoholic fermentation and to strategies for altering redox-cofactor coupling in carbon and nitrogen metabolism that aim to reduce or eliminate the role of glycerol formation in anaerobic redox metabolism. In addition to providing an overview of scientific advances we discuss context dependency, theoretical impact and potential for industrial application of different proposed and developed strategies.

Original languageEnglish
Pages (from-to)554-566
JournalSynthetic and Systems Biotechnology
Volume7
Issue number1
DOIs
Publication statusPublished - 2022

Keywords

  • Biofuels
  • Energy metabolism
  • Metabolic engineering
  • Redox metabolism
  • Saccharomyces cerevisiae
  • Synthetic biology

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