Pathway engineering strategies for improved product yield in yeast-based industrial ethanol production

Aafke C.A. van Aalst; Sophie C. de Valk; Walter M. van Gulik; Mickel L.A. Jansen; Jack T. Pronk; Robert Mans

doi:10.1016/j.synbio.2021.12.010

Pathway engineering strategies for improved product yield in yeast-based industrial ethanol production

Aafke C.A. van Aalst, Sophie C. de Valk, Walter M. van Gulik, Mickel L.A. Jansen, Jack T. Pronk, Robert Mans^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Scientific › peer-review

13 Citations (Scopus)

172 Downloads (Pure)

Abstract

Product yield on carbohydrate feedstocks is a key performance indicator for industrial ethanol production with the yeast Saccharomyces cerevisiae. This paper reviews pathway engineering strategies for improving ethanol yield on glucose and/or sucrose in anaerobic cultures of this yeast by altering the ratio of ethanol production, yeast growth and glycerol formation. Particular attention is paid to strategies aimed at altering energy coupling of alcoholic fermentation and to strategies for altering redox-cofactor coupling in carbon and nitrogen metabolism that aim to reduce or eliminate the role of glycerol formation in anaerobic redox metabolism. In addition to providing an overview of scientific advances we discuss context dependency, theoretical impact and potential for industrial application of different proposed and developed strategies.

Original language	English
Pages (from-to)	554-566
Journal	Synthetic and Systems Biotechnology
Volume	7
Issue number	1
DOIs	https://doi.org/10.1016/j.synbio.2021.12.010
Publication status	Published - 2022

Keywords

Biofuels
Energy metabolism
Metabolic engineering
Redox metabolism
Saccharomyces cerevisiae
Synthetic biology

Access to Document

10.1016/j.synbio.2021.12.010

1-s2.0-S2405805X21000995-mainFinal published version, 2.71 MBLicence: CC BY

Cite this

@article{719f0bdd932b431f8b600b8504c8426a,

title = "Pathway engineering strategies for improved product yield in yeast-based industrial ethanol production",

abstract = "Product yield on carbohydrate feedstocks is a key performance indicator for industrial ethanol production with the yeast Saccharomyces cerevisiae. This paper reviews pathway engineering strategies for improving ethanol yield on glucose and/or sucrose in anaerobic cultures of this yeast by altering the ratio of ethanol production, yeast growth and glycerol formation. Particular attention is paid to strategies aimed at altering energy coupling of alcoholic fermentation and to strategies for altering redox-cofactor coupling in carbon and nitrogen metabolism that aim to reduce or eliminate the role of glycerol formation in anaerobic redox metabolism. In addition to providing an overview of scientific advances we discuss context dependency, theoretical impact and potential for industrial application of different proposed and developed strategies.",

keywords = "Biofuels, Energy metabolism, Metabolic engineering, Redox metabolism, Saccharomyces cerevisiae, Synthetic biology",

author = "{van Aalst}, {Aafke C.A.} and {de Valk}, {Sophie C.} and {van Gulik}, {Walter M.} and Jansen, {Mickel L.A.} and Pronk, {Jack T.} and Robert Mans",

year = "2022",

doi = "10.1016/j.synbio.2021.12.010",

language = "English",

volume = "7",

pages = "554--566",

journal = "Synthetic and Systems Biotechnology",

issn = "2405-805X",

publisher = "KeAi Communications Co.",

number = "1",

}

TY - JOUR

T1 - Pathway engineering strategies for improved product yield in yeast-based industrial ethanol production

AU - van Aalst, Aafke C.A.

AU - de Valk, Sophie C.

AU - van Gulik, Walter M.

AU - Jansen, Mickel L.A.

AU - Pronk, Jack T.

AU - Mans, Robert

PY - 2022

Y1 - 2022

N2 - Product yield on carbohydrate feedstocks is a key performance indicator for industrial ethanol production with the yeast Saccharomyces cerevisiae. This paper reviews pathway engineering strategies for improving ethanol yield on glucose and/or sucrose in anaerobic cultures of this yeast by altering the ratio of ethanol production, yeast growth and glycerol formation. Particular attention is paid to strategies aimed at altering energy coupling of alcoholic fermentation and to strategies for altering redox-cofactor coupling in carbon and nitrogen metabolism that aim to reduce or eliminate the role of glycerol formation in anaerobic redox metabolism. In addition to providing an overview of scientific advances we discuss context dependency, theoretical impact and potential for industrial application of different proposed and developed strategies.

AB - Product yield on carbohydrate feedstocks is a key performance indicator for industrial ethanol production with the yeast Saccharomyces cerevisiae. This paper reviews pathway engineering strategies for improving ethanol yield on glucose and/or sucrose in anaerobic cultures of this yeast by altering the ratio of ethanol production, yeast growth and glycerol formation. Particular attention is paid to strategies aimed at altering energy coupling of alcoholic fermentation and to strategies for altering redox-cofactor coupling in carbon and nitrogen metabolism that aim to reduce or eliminate the role of glycerol formation in anaerobic redox metabolism. In addition to providing an overview of scientific advances we discuss context dependency, theoretical impact and potential for industrial application of different proposed and developed strategies.

KW - Biofuels

KW - Energy metabolism

KW - Metabolic engineering

KW - Redox metabolism

KW - Saccharomyces cerevisiae

KW - Synthetic biology

UR - http://www.scopus.com/inward/record.url?scp=85123194037&partnerID=8YFLogxK

U2 - 10.1016/j.synbio.2021.12.010

DO - 10.1016/j.synbio.2021.12.010

M3 - Article

AN - SCOPUS:85123194037

SN - 2405-805X

VL - 7

SP - 554

EP - 566

JO - Synthetic and Systems Biotechnology

JF - Synthetic and Systems Biotechnology

IS - 1

ER -

Pathway engineering strategies for improved product yield in yeast-based industrial ethanol production

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Engineering and integration of pathways for anaerobic redox-cofactor balancing in yeast

Cite this