TY - JOUR
T1 - PDGFRβ+ cells play a dual role as hematopoietic precursors and niche cells during mouse ontogeny
AU - Sá da Bandeira, Diana
AU - Kilpatrick, Alastair Morris
AU - Marques, Madalena
AU - Gomez-Salazar, Mario
AU - Ventura, Telma
AU - Gonzalez, Zaniah Nashira
AU - Stefancova, Dorota
AU - Rossi, Fiona
AU - Vink, Chris Sebastiaan
AU - More Authors, null
PY - 2022
Y1 - 2022
N2 - Hematopoietic stem cell (HSC) generation in the aorta-gonad-mesonephros region requires HSC specification signals from the surrounding microenvironment. In zebrafish, PDGF-B/PDGFRβ signaling controls hematopoietic stem/progenitor cell (HSPC) generation and is required in the HSC specification niche. Little is known about murine HSPC specification in vivo and whether PDGF-B/PDGFRβ is involved. Here, we show that PDGFRβ is expressed in distinct perivascular stromal cell layers surrounding the mid-gestation dorsal aorta, and its deletion impairs hematopoiesis. We demonstrate that PDGFRβ+ cells play a dual role in murine hematopoiesis. They act in the aortic niche to support HSPCs, and in addition, PDGFRβ+ embryonic precursors give rise to a subset of HSPCs that persist into adulthood. These findings provide crucial information for the controlled production of HSPCs in vitro.
AB - Hematopoietic stem cell (HSC) generation in the aorta-gonad-mesonephros region requires HSC specification signals from the surrounding microenvironment. In zebrafish, PDGF-B/PDGFRβ signaling controls hematopoietic stem/progenitor cell (HSPC) generation and is required in the HSC specification niche. Little is known about murine HSPC specification in vivo and whether PDGF-B/PDGFRβ is involved. Here, we show that PDGFRβ is expressed in distinct perivascular stromal cell layers surrounding the mid-gestation dorsal aorta, and its deletion impairs hematopoiesis. We demonstrate that PDGFRβ+ cells play a dual role in murine hematopoiesis. They act in the aortic niche to support HSPCs, and in addition, PDGFRβ+ embryonic precursors give rise to a subset of HSPCs that persist into adulthood. These findings provide crucial information for the controlled production of HSPCs in vitro.
KW - AGM single-cell RNA-sequencing
KW - Bmp4
KW - CP: Developmental biology
KW - CP: Stem cell research
KW - hematopoietic niche
KW - HSPC precursor
KW - MSCs
KW - osteogenesis
KW - PDGFRβ
KW - pericytes
KW - VSMCs
UR - http://www.scopus.com/inward/record.url?scp=85134780236&partnerID=8YFLogxK
U2 - 10.1016/j.celrep.2022.111114
DO - 10.1016/j.celrep.2022.111114
M3 - Article
C2 - 35858557
AN - SCOPUS:85134780236
SN - 2211-1247
VL - 40
JO - Cell Reports
JF - Cell Reports
IS - 3
M1 - 111114
ER -