pH-gradient chromatofocusing of proteins on a chip

Hoon Suk Rho, Alexander T. Hanke, Marcel Ottens, Han Gardeniers*

*Corresponding author for this work

Research output: Chapter in Book/Conference proceedings/Edited volumeConference contributionScientificpeer-review

Abstract

We present a novel microfluidic system for the pH-gradient focusing of proteins with the integration of 16 parallel micro-mixers, a micro-column, and a multiplexer. In this work we successfully achieved the creation of 16 non-linear gradients and the generation of a solid-phase micro-column for the realization of anion exchange chromatography on a single chip. With the device we demonstrated the separation of a protein mixture of R-phycoerythrin and FITC-BSA based on pH-gradient chromatofocusing.

Original languageEnglish
Title of host publicationMicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages245-247
Number of pages3
ISBN (Electronic)9780979806483
Publication statusPublished - 2015
Event19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of
Duration: 25 Oct 201529 Oct 2015

Conference

Conference19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
CountryKorea, Republic of
CityGyeongju
Period25/10/1529/10/15

Keywords

  • Chromatofocusing
  • Micro-column
  • Microfluidics
  • PH gradient

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