Abstract
The pilot-scale production of the peroxygenase from Agrocybe aegerita (rAaeUPO) is demonstrated. In a fed-batch fermentation of the recombinant Pichia pastoris, the enzyme was secreted into the culture medium to a final concentration of 0.29 g L-1 corresponding to 735 g of the peroxygenase in 2500 L of the fermentation broth after 6 days. Due to nonoptimized downstream processing, only 170 g of the enzyme has been isolated. The preparative usefulness of the so-obtained enzyme preparation has been demonstrated at a semipreparative scale (100 mL) as an example of the stereoselective hydroxylation of ethyl benzene. Using an adjusted H2O2 feed rate, linear product formation was observed for 7 days, producing more than 5 g L-1 (R)-1-phenyl ethanol. The biocatalyst performed more than 340.000 catalytic turnovers (942 g of the product per gram of rAaeUPO).
Original language | English |
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Pages (from-to) | 1414-1418 |
Number of pages | 5 |
Journal | Organic Process Research and Development |
Volume | 25 |
Issue number | 6 |
DOIs | |
Publication status | Published - 2021 |
Keywords
- Biocatalysis
- Hydroxylation
- Peroxygenases
- Pilot-scale fermentation
- Specific oxyfunctionalization