Pilot-scale production of peroxygenase from agrocybe aegerita

Fabio Tonin, Florian Tieves, Sébastien Willot, Anouska Van Troost, Remco Van Oosten, Stefaan Breestraat, Sander Van Pelt, Miguel Alcalde, Frank Hollmann

Research output: Contribution to journalArticleScientificpeer-review

2 Citations (Scopus)
6 Downloads (Pure)

Abstract

The pilot-scale production of the peroxygenase from Agrocybe aegerita (rAaeUPO) is demonstrated. In a fed-batch fermentation of the recombinant Pichia pastoris, the enzyme was secreted into the culture medium to a final concentration of 0.29 g L-1 corresponding to 735 g of the peroxygenase in 2500 L of the fermentation broth after 6 days. Due to nonoptimized downstream processing, only 170 g of the enzyme has been isolated. The preparative usefulness of the so-obtained enzyme preparation has been demonstrated at a semipreparative scale (100 mL) as an example of the stereoselective hydroxylation of ethyl benzene. Using an adjusted H2O2 feed rate, linear product formation was observed for 7 days, producing more than 5 g L-1 (R)-1-phenyl ethanol. The biocatalyst performed more than 340.000 catalytic turnovers (942 g of the product per gram of rAaeUPO).

Original languageEnglish
Pages (from-to)1414-1418
Number of pages5
JournalOrganic Process Research and Development
Volume25
Issue number6
DOIs
Publication statusPublished - 2021

Keywords

  • Biocatalysis
  • Hydroxylation
  • Peroxygenases
  • Pilot-scale fermentation
  • Specific oxyfunctionalization

Fingerprint

Dive into the research topics of 'Pilot-scale production of peroxygenase from agrocybe aegerita'. Together they form a unique fingerprint.

Cite this