Plug-and-play adaptive optics for commercial laser scanning fluorescence microscopes based on an adaptive lens

P. Pozzi, M. Quintavalla, A. B. Wong, J. G.G. Borst, S. Bonora, M. Verhaegen

Research output: Contribution to journalArticleScientificpeer-review

Abstract

In this Letter, we present a solution for simple implementation of adaptive optics in any existing laser scanning fluorescence microscope. Adaptive optics are implemented by the introduction of a multiactuator adaptive lens between the microscope body and the objective lens. Correction is performed with a sensorless method by optimizing the quality of the images presented on screen by the microscope software. We present the results acquired on both a commercial linear excitation confocal microscope and a custom-made multiphoton excitation microscope.

Original languageEnglish
Pages (from-to)3585-3588
JournalOptics Letters
Volume45
Issue number13
DOIs
Publication statusPublished - 2020

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