Abstract
In this Letter, we present a solution for simple implementation of adaptive optics in any existing laser scanning fluorescence microscope. Adaptive optics are implemented by the introduction of a multiactuator adaptive lens between the microscope body and the objective lens. Correction is performed with a sensorless method by optimizing the quality of the images presented on screen by the microscope software. We present the results acquired on both a commercial linear excitation confocal microscope and a custom-made multiphoton excitation microscope.
Original language | English |
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Pages (from-to) | 3585-3588 |
Journal | Optics Letters |
Volume | 45 |
Issue number | 13 |
DOIs | |
Publication status | Published - 2020 |