TY - JOUR
T1 - Point spread function based image reconstruction in optical projection tomography
AU - Trull, Anna
AU - van der Horst, Jelle
AU - Palenstijn, Willem Jan
AU - van Vliet, Lucas
AU - van Leeuwen, Tristan
AU - Kalkman, Jeroen
N1 - Accepted Author Manuscript
PY - 2017
Y1 - 2017
N2 - As a result of the shallow depth of focus of the optical imaging system, the use of standard filtered back projection in optical projection tomography causes space-variant tangential blurring that increases with the distance to the rotation axis. We present a novel optical tomographic image reconstruction technique that incorporates the point spread function of the imaging lens in an iterative reconstruction. The technique is demonstrated using numerical simulations, tested on experimental optical projection tomography data of single fluorescent beads, and applied to high-resolution emission optical projection tomography imaging of an entire zebrafish larva. Compared to filtered back projection our results show greatly reduced radial and tangential blurring over the entire 5.2x5.2 mm2 field of view, and a significantly improved signal to noise ratio.
AB - As a result of the shallow depth of focus of the optical imaging system, the use of standard filtered back projection in optical projection tomography causes space-variant tangential blurring that increases with the distance to the rotation axis. We present a novel optical tomographic image reconstruction technique that incorporates the point spread function of the imaging lens in an iterative reconstruction. The technique is demonstrated using numerical simulations, tested on experimental optical projection tomography data of single fluorescent beads, and applied to high-resolution emission optical projection tomography imaging of an entire zebrafish larva. Compared to filtered back projection our results show greatly reduced radial and tangential blurring over the entire 5.2x5.2 mm2 field of view, and a significantly improved signal to noise ratio.
KW - Image reconstruction techniques
KW - Inverse problems
KW - omographic image processing
UR - http://resolver.tudelft.nl/uuid:4ed74e00-be0e-4c77-b0b9-74829ce848c7
U2 - 10.1088/1361-6560/aa8945
DO - 10.1088/1361-6560/aa8945
M3 - Article
SN - 0031-9155
VL - 62
SP - 7784
EP - 7797
JO - Physics in Medicine and Biology
JF - Physics in Medicine and Biology
IS - 19
ER -