Practical guidelines for implementing adaptive optics in fluorescence microscopy

Dean Wilding, Paolo Pozzi, Oleg Soloviev, Gleb Vdovin, Michel Verhaegen

Research output: Chapter in Book/Conference proceedings/Edited volumeConference contributionScientificpeer-review

1 Citation (Scopus)
12 Downloads (Pure)

Abstract

In life sciences, interest in the microscopic imaging of increasingly complex three dimensional samples, such as cell spheroids, zebrafish embryos, and in vivo applications in small animals, is growing quickly. Due to the increasing complexity of samples, more and more life scientists are considering the implementation of adaptive optics in their experimental setups. While several approaches to adaptive optics in microscopy have been reported, it is often difficult and confusing for the microscopist to choose from the array of techniques and equipment. In this poster presentation we offer a small guide to adaptive optics providing general guidelines for successful adaptive optics implementation.

Original languageEnglish
Title of host publicationProceedings of SPIE
Subtitle of host publicationAdaptive Optics and Wavefront Control for Biological Systems IV
EditorsT.G. Bifano, J. Kubby, S. Gigan
Place of PublicationBellingham, WA, USA
PublisherSPIE
Number of pages13
Volume10502
ISBN (Electronic)9781510614895
DOIs
Publication statusPublished - 2018
EventSPIE BIOS: Adaptive Optics and Wavefront Control for Biological Systems IV 2018 - San Francisco, United States
Duration: 27 Jan 201829 Jan 2018

Publication series

NameProceedings of SPIE
Volume10502

Conference

ConferenceSPIE BIOS
CountryUnited States
CitySan Francisco
Period27/01/1829/01/18

Keywords

  • Adaptive optics
  • fluorescence microscopy
  • wavefront sensing

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  • Cite this

    Wilding, D., Pozzi, P., Soloviev, O., Vdovin, G., & Verhaegen, M. (2018). Practical guidelines for implementing adaptive optics in fluorescence microscopy. In T. G. Bifano, J. Kubby, & S. Gigan (Eds.), Proceedings of SPIE: Adaptive Optics and Wavefront Control for Biological Systems IV (Vol. 10502). [105021F] (Proceedings of SPIE; Vol. 10502). SPIE. https://doi.org/10.1117/12.2287647