TY - JOUR
T1 - Probing the salt dependence of the torsional stiffness of DNA by multiplexed magnetic torque tweezers
AU - Kriegel, Franziska
AU - Ermann, Niklas
AU - Forbes, Ruaridh
AU - Dulin, David
AU - Dekker, Nynke H.
AU - Lipfert, Jan
PY - 2017
Y1 - 2017
N2 - The mechanical properties of DNA fundamentally constrain and enable the storage and transmission of genetic information and its use in DNA nanotechnology. Many properties of DNA depend on the ionic environment due to its highly charged backbone. In particular, both theoretical analyses and direct singlemolecule experiments have shown its bending stiffness to depend on salt concentration. In contrast, the salt-dependence of the twist stiffness of DNA is much less explored. Here, we employ optimized multiplexed magnetic torque tweezers to study the torsional stiffness of DNA under varying salt conditions as a function of stretching force. At low forces (<3 pN), the effective torsional stiffness is ∼10% smaller for high salt conditions (500 mM NaCl or 10 mM MgCl2) compared to lower salt concentrations (20 mM NaCl and 100 mM NaCl). These differences, however, can be accounted for by taking into account the known salt dependence of the bending stiffness. In addition, the measured high-force (6.5 pN) torsional stiffness values of C = 103 ± 4 nm are identical, within experimental errors, for all tested salt concentration, suggesting that the intrinsic torsional stiffness of DNA does not depend on salt.
AB - The mechanical properties of DNA fundamentally constrain and enable the storage and transmission of genetic information and its use in DNA nanotechnology. Many properties of DNA depend on the ionic environment due to its highly charged backbone. In particular, both theoretical analyses and direct singlemolecule experiments have shown its bending stiffness to depend on salt concentration. In contrast, the salt-dependence of the twist stiffness of DNA is much less explored. Here, we employ optimized multiplexed magnetic torque tweezers to study the torsional stiffness of DNA under varying salt conditions as a function of stretching force. At low forces (<3 pN), the effective torsional stiffness is ∼10% smaller for high salt conditions (500 mM NaCl or 10 mM MgCl2) compared to lower salt concentrations (20 mM NaCl and 100 mM NaCl). These differences, however, can be accounted for by taking into account the known salt dependence of the bending stiffness. In addition, the measured high-force (6.5 pN) torsional stiffness values of C = 103 ± 4 nm are identical, within experimental errors, for all tested salt concentration, suggesting that the intrinsic torsional stiffness of DNA does not depend on salt.
UR - http://resolver.tudelft.nl/uuid:182d1a75-3a30-49f3-a0b1-de68f707e6e6
UR - http://www.scopus.com/inward/record.url?scp=85022030447&partnerID=8YFLogxK
U2 - 10.1093/nar/gkx280
DO - 10.1093/nar/gkx280
M3 - Article
C2 - 28460037
SN - 0305-1048
VL - 45
SP - 5920
EP - 5929
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 10
ER -