TY - JOUR
T1 - Process conditions affect properties and outcomes of polyhydroxyalkanoate accumulation in municipal activated sludge
AU - Estévez-Alonso, Ángel
AU - Altamira-Algarra, Beatriz
AU - Arnau-Segarra, César
AU - van Loosdrecht, Mark C.M.
AU - Kleerebezem, Robbert
AU - Werker, Alan
PY - 2022
Y1 - 2022
N2 - The developments of mixed culture polyhydroxyalkanoate production has been directed to maximize the biomass PHA content with limited attention to polymer quality. Direct comparison of PHA accumulation literature is challenging, and even regularly contradicting in reported results, due to underlying differences that are not well expressed. A study was undertaken to systematically compare the commonly reported process conditions for PHA accumulation by full-scale municipal activated sludge. A biomass acclimation step combined with a pulse-wise feeding strategy resulted in maximum average PHA contents and product yields. pH control and active nitrification did not result in observable effects on the PHA productivity. Under these conditions a high molecular weight polymer (1536 ± 221 kDa) can be produced. Polymer extraction recoveries were influenced by the PHA molecular weight. A standard protocol for an activated sludge PHA accumulation test including downstream processing and standardized extraction has been developed and is available as supplementary material.
AB - The developments of mixed culture polyhydroxyalkanoate production has been directed to maximize the biomass PHA content with limited attention to polymer quality. Direct comparison of PHA accumulation literature is challenging, and even regularly contradicting in reported results, due to underlying differences that are not well expressed. A study was undertaken to systematically compare the commonly reported process conditions for PHA accumulation by full-scale municipal activated sludge. A biomass acclimation step combined with a pulse-wise feeding strategy resulted in maximum average PHA contents and product yields. pH control and active nitrification did not result in observable effects on the PHA productivity. Under these conditions a high molecular weight polymer (1536 ± 221 kDa) can be produced. Polymer extraction recoveries were influenced by the PHA molecular weight. A standard protocol for an activated sludge PHA accumulation test including downstream processing and standardized extraction has been developed and is available as supplementary material.
KW - Bioplastics
KW - Feeding strategy
KW - Molecular weight
KW - Polyhydroxyalkanoate
KW - Waste activated sludge
UR - http://www.scopus.com/inward/record.url?scp=85139048161&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2022.128035
DO - 10.1016/j.biortech.2022.128035
M3 - Article
AN - SCOPUS:85139048161
VL - 364
JO - Bioresource Technology
JF - Bioresource Technology
SN - 0960-8524
M1 - 128035
ER -
