Production and immobilization of lipase PCL and its application in synthesis of α‐linolenic acid‐rich diacylglycerol

Nan Liu, Daoming Liu, Weifei Wang, Frank Hollmann, Long Xu, Yunjian Ma, Bo Yang, Weidong Bai, Xiaotao Sun, Yonghua Wang

Research output: Contribution to journalArticleScientificpeer-review

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α‐Linolenic acid‐rich diacylglycerol has been demonstrated with promising health promotion functions. This study examined the production, immobilization of lipase PCL, and its application in the synthesis of diacylglycerol by esterification of α‐linolenic acid with glycerol. The resin ECR8806 was selected as an effective support for the immobilization of lipase PCL. Fourier transform infrared and Laser scanning confocal microscope analysis proved that the lipase was successfully immobilized on the resin. Compared with the free PCL, the immobilized one exhibited higher temperature tolerance. Under optimized reaction conditions, a DAG content of 54.49% were obtained. After further purified by molecular distillation, the purity of DAG was up to 99.28%. During esterification, the immobilized PCL was quite stable and retained more than 91.60% of its initial activity after 10 cycles. These new findings on the immobilized PCL will make it to be a prospective enzyme in oils and fats industry.
Original languageEnglish
Article numbere12574
Number of pages9
JournalJournal of Food Biochemistry
Publication statusPublished - 2018

Bibliographical note

Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.


  • diacylglycerol
  • esterification
  • immobilization
  • reusability
  • a-Linolenic acid


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