Protease and phenol production of Cynara cardunculus L. Cell suspension in a chemostat

M. E. Lima Costa, W. M. Van Gulik, J. G. Ten Hoopen, M. S.S. Pais, J. M.S. Cabral

Research output: Contribution to journalArticleScientificpeer-review

11 Citations (Scopus)

Abstract

Proteases from Cynara cardunculus L. are used in cheese-making. The growth of C. cardunculus cell suspension culture and the production of proteases and phenols were studied in sucrose-limited chemostat cultures at two dilution rates. Biomass yields and biomass and protease productivities in chemostat cultures were compared with those in batch cultures. C. cardunculus cells showed a high ability to adapt to chemostat conditions. Phenolic production was not observed in the steady-state cultures of C. cardunculus cell cultures in contrast to batch cultures. Protease production was linked to the exponential growth phase and was higher and more stable in sucrose-limited chemostats than in batch culture.

Original languageEnglish
Pages (from-to)493-500
JournalEnzyme and Microbial Technology: biotechnology research and reviews
Volume19
Issue number7
DOIs
Publication statusPublished - 1996

Keywords

  • Chemostat
  • Cynara cardunculus
  • Phenols
  • Plant cell suspension
  • Proteases

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