Protocol for multimodal analysis of human kidney tissue by imaging mass spectrometry and CODEX multiplexed immunofluorescence

Elizabeth K. Neumann, Nathan Heath Patterson, Jamie L. Allen, Lukasz G. Migas, Haichun Yang, Maya Brewer, David M. Anderson, Jennifer Harvey, Raymond C. Harris, Raf Van de Plas, More Authors

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

Here, we describe the preservation and preparation of human kidney tissue for interrogation by histopathology, imaging mass spectrometry, and multiplexed immunofluorescence. Custom image registration and integration techniques are used to create cellular and molecular atlases of this organ system. Through careful optimization, we ensure high-quality and reproducible datasets suitable for cross-patient comparisons that are essential to understanding human health and disease. Moreover, each of these steps can be adapted to other organ systems or diseases, enabling additional atlas efforts.

Original languageEnglish
Article number100747
Number of pages21
JournalSTAR Protocols
Volume2
Issue number3
DOIs
Publication statusPublished - 2021

Keywords

  • Antibody
  • Chemistry
  • Health Sciences
  • Mass Spectrometry
  • Metabolomics
  • Microscopy

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