Research output per year
Research output per year
Jos de Wit*, Sebastian Tonn, Guido van den Ackerveken, Jeroen Kalkman
Research output: Contribution to journal › Article › Scientific › peer-review
Optical coherence tomography (OCT) can be a valuable imaging tool for in vivo and label-free digital plant phenotyping. However, for imaging leaves, air-filled cavities limit the penetration depth and reduce the image quality. Moreover, up to now quantification of leaf morphology with OCT has been done in one-dimensional or two-dimensional images only, and has often been limited to relative measurements. In this paper, we demonstrate a significant increase in OCT imaging depth and image quality by infiltrating the leaf air spaces with water. In the obtained high-quality OCT images the top and bottom surface of the leaf are digitally segmented. Moreover, high-quality en face images of the leaf are obtained from numerically flattened leaves. Segmentation in three-dimensional OCT images is used to quantify the spatially resolved leaf thickness. Based on a segmented leaf image, the refractive index of an infiltrated leaf is measured to be 1.345 ± 0.004, deviating only 1.2% from that of pure water. Using the refractive index and a correction for refraction effects at the air-leaf interface, we quantitatively mapped the leaf thickness. The results show that OCT is an efficient and promising technique for quantitative phenotyping on leaf and tissue level.
Original language | English |
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Pages (from-to) | 10304-10311 |
Journal | Applied Optics |
Volume | 59 |
Issue number | 33 |
DOIs | |
Publication status | Published - 2020 |
Research output: Thesis › Dissertation (TU Delft)