Quantification of saquinavir from lysates of peripheral blood mononuclear cells using Microarrays and standard MALDI-TOF-MS

Martin Pabst, Stephan Rupert Fagerer, Rudolf Köhling, Klaus Eyer, Jasmin Krismer, Konstantins Jefimovs, Alfredo Jesus Ibáñez, Renato Zenobi

Research output: Contribution to journalArticleScientificpeer-review

3 Citations (Scopus)

Abstract

Drug monitoring is usually performed by liquid chromatography coupled with optical detection or electrospray ionization mass spectrometry. More recently, matrix-assisted laser desorption/ionization (MALDI) in combination with triple quadrupole or Fourier-transform (FT) mass analyzers has also been reported to allow accurate quantification. Here, we present a strategy that employs standard MALDI time-of-flight (TOF) mass spectrometry (MS) for the sensitive and accurate quantification of saquinavir from an extract of blood peripheral mononuclear cells. Unambiguous identification of saquinavir in the mass spectra was possible because of using internal mass calibration and by an overall low chemical noise in the low mass range. Exact mass determination of the constant background peaks of the cell extract, which were used for recalibration, was performed by an initial MALDI-FT-MS analysis. Fast and multiplexed sample analysis was enabled by microarray technology, which provided 10 replicates in the lower nL range for each sample in parallel lanes on a chip. In order to validate the method, we employed various statistical tests, such as confidence intervals for linear regressions, three quality control samples, and inverse confidence limits of the estimated concentration ratios. [Figure not available: see fulltext.]

Original languageEnglish
Pages (from-to)1083-1086
JournalJournal of the American Society for Mass Spectrometry
Volume25
Issue number6
DOIs
Publication statusPublished - 2014
Externally publishedYes

Keywords

  • Antiretroviral drugs
  • Cell lysate analysis
  • Microarrays for mass spectrometry (MAMS)
  • Peripheral blood mononuclear cells
  • Quantitative MALDI-MS
  • Saquinavir

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