TY - JOUR
T1 - Quantitative analysis of myofiber type composition in human and mouse skeletal muscles
AU - Abbassi-Daloii, Tooba
AU - el Abdellaoui, Salma
AU - Kan, Hermien E.
AU - van den Akker, Erik
AU - ’t Hoen, Peter A.C.
AU - Raz, Vered
AU - Voortman, Lenard M.
PY - 2023
Y1 - 2023
N2 - Skeletal muscles are composed of different myofiber types characterized by the expression of myosin heavy chain isoforms, which can be affected by physical activity, aging, and pathological conditions. Here, we present a step-by-step high-throughput semi-automated approach for performing myofiber type quantification of entire human or mouse muscle tissue sections, including immunofluorescence staining, image acquisition, processing, and quantification. For complete details on the use and execution of this protocol, please refer to Abbassi-Daloii et al. (2022).1
AB - Skeletal muscles are composed of different myofiber types characterized by the expression of myosin heavy chain isoforms, which can be affected by physical activity, aging, and pathological conditions. Here, we present a step-by-step high-throughput semi-automated approach for performing myofiber type quantification of entire human or mouse muscle tissue sections, including immunofluorescence staining, image acquisition, processing, and quantification. For complete details on the use and execution of this protocol, please refer to Abbassi-Daloii et al. (2022).1
KW - High-Throughput Screening
KW - Microscopy
UR - http://www.scopus.com/inward/record.url?scp=85147204922&partnerID=8YFLogxK
U2 - 10.1016/j.xpro.2023.102075
DO - 10.1016/j.xpro.2023.102075
M3 - Article
AN - SCOPUS:85147204922
SN - 2666-1667
VL - 4
JO - STAR Protocols
JF - STAR Protocols
IS - 1
M1 - 102075
ER -