Recent developments in the use of peroxygenases – Exploring their high potential in selective oxyfunctionalisations

Markus Hobisch; Dirk Holtmann; Patricia Gomez de Santos; Miguel Alcalde; Frank Hollmann; Selin Kara

doi:10.1016/j.biotechadv.2020.107615

Recent developments in the use of peroxygenases – Exploring their high potential in selective oxyfunctionalisations

Markus Hobisch, Dirk Holtmann, Patricia Gomez de Santos, Miguel Alcalde, Frank Hollmann, Selin Kara^*

^*Corresponding author for this work

BT/Biocatalysis

Research output: Contribution to journal › Review article › peer-review

91 Citations (Scopus)

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Abstract

Peroxygenases are an emerging new class of enzymes allowing selective oxyfunctionalisation reactions in a cofactor-independent way different from well-known P450 monooxygenases. Herein, we focused on recent developments from organic synthesis, molecular biotechnology and reaction engineering viewpoints that are devoted to bring these enzymes in industrial applications. This covers natural diversity from different sources, protein engineering strategies for expression, substrate scope, activity and selectivity, stabilisation of enzymes via immobilisation, and the use of peroxygenases in low water media. We believe that peroxygenases have much to offer for selective oxyfunctionalisations and we have much to study to explore the full potential of these versatile biocatalysts in organic synthesis.

Original language	English
Article number	107615
Number of pages	13
Journal	Biotechnology Advances
Volume	51
DOIs	https://doi.org/10.1016/j.biotechadv.2020.107615
Publication status	Published - 2021

Keywords

Fungal enzyme
Oxidation chemistry
Oxyfunctionalisation
Oxygenase
Peroxygenase

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10.1016/j.biotechadv.2020.107615

1-s2.0-S0734975020301178-mainFinal published version, 4.84 MBLicence: CC BY-NC-ND

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abstract = "Peroxygenases are an emerging new class of enzymes allowing selective oxyfunctionalisation reactions in a cofactor-independent way different from well-known P450 monooxygenases. Herein, we focused on recent developments from organic synthesis, molecular biotechnology and reaction engineering viewpoints that are devoted to bring these enzymes in industrial applications. This covers natural diversity from different sources, protein engineering strategies for expression, substrate scope, activity and selectivity, stabilisation of enzymes via immobilisation, and the use of peroxygenases in low water media. We believe that peroxygenases have much to offer for selective oxyfunctionalisations and we have much to study to explore the full potential of these versatile biocatalysts in organic synthesis.",

keywords = "Fungal enzyme, Oxidation chemistry, Oxyfunctionalisation, Oxygenase, Peroxygenase",

author = "Markus Hobisch and Dirk Holtmann and {Gomez de Santos}, Patricia and Miguel Alcalde and Frank Hollmann and Selin Kara",

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AU - Hobisch, Markus

AU - Holtmann, Dirk

AU - Gomez de Santos, Patricia

AU - Alcalde, Miguel

AU - Hollmann, Frank

AU - Kara, Selin

PY - 2021

Y1 - 2021

N2 - Peroxygenases are an emerging new class of enzymes allowing selective oxyfunctionalisation reactions in a cofactor-independent way different from well-known P450 monooxygenases. Herein, we focused on recent developments from organic synthesis, molecular biotechnology and reaction engineering viewpoints that are devoted to bring these enzymes in industrial applications. This covers natural diversity from different sources, protein engineering strategies for expression, substrate scope, activity and selectivity, stabilisation of enzymes via immobilisation, and the use of peroxygenases in low water media. We believe that peroxygenases have much to offer for selective oxyfunctionalisations and we have much to study to explore the full potential of these versatile biocatalysts in organic synthesis.

AB - Peroxygenases are an emerging new class of enzymes allowing selective oxyfunctionalisation reactions in a cofactor-independent way different from well-known P450 monooxygenases. Herein, we focused on recent developments from organic synthesis, molecular biotechnology and reaction engineering viewpoints that are devoted to bring these enzymes in industrial applications. This covers natural diversity from different sources, protein engineering strategies for expression, substrate scope, activity and selectivity, stabilisation of enzymes via immobilisation, and the use of peroxygenases in low water media. We believe that peroxygenases have much to offer for selective oxyfunctionalisations and we have much to study to explore the full potential of these versatile biocatalysts in organic synthesis.

KW - Fungal enzyme

KW - Oxidation chemistry

KW - Oxyfunctionalisation

KW - Oxygenase

KW - Peroxygenase

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DO - 10.1016/j.biotechadv.2020.107615

M3 - Review article

AN - SCOPUS:85089826545

SN - 0734-9750

VL - 51

JO - Biotechnology Advances

JF - Biotechnology Advances

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ER -

Recent developments in the use of peroxygenases – Exploring their high potential in selective oxyfunctionalisations

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Keywords

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