Reconstitution of isotopically labeled ribosomal protein L29 in the 50S large ribosomal subunit for solution-state and solid-state NMR

Emeline Barbet-Massin, Eli van der Sluis, Joanna Musial, Roland Beckmann, Bernd Reif

    Research output: Chapter in Book/Conference proceedings/Edited volumeChapterScientificpeer-review

    3 Citations (Scopus)

    Abstract

    Solid-state nuclear magnetic resonance (NMR) has recently emerged as a method of choice to study structural and dynamic properties of large biomolecular complexes at atomic resolution. Indeed, recent technological and methodological developments have enabled the study of ever more complex systems in the solid-state. However, to explore multicomponent protein complexes by NMR, specific labeling schemes need to be developed that are dependent on the biological question to be answered. We show here how to reconstitute an isotopically labeled protein within the unlabeled 50S or 70S ribosomal subunit. In particular, we focus on the 63-residue ribosomal protein L29 (~7 kDa), which is located at the exit of the tunnel of the large 50S ribosomal subunit (~1.5 MDa). The aim of this work is the preparation of a suitable sample to investigate allosteric conformational changes in a ribosomal protein that are induced by the nascent polypeptide chain and that trigger the interaction with different chaperones (e.g., trigger factor or SRP).

    Original languageEnglish
    Title of host publicationMethods in Molecular Biology
    PublisherSpringer
    Pages87-100
    Number of pages14
    Volume1764
    DOIs
    Publication statusPublished - 1 Jan 2018

    Publication series

    NameMethods in Molecular Biology
    Volume1764
    ISSN (Print)1064-3745

    Keywords

    • Isotope labeling
    • MAS solid-state NMR spectroscopy
    • Protein complex reconstitution

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