Recording Neuronal Activity On Chip with Segmented 3D Microelectrode Arrays

Nele Revyn; Michel H.Y. Hu; Jean-Philippe Frimat; Bjorn de Wagenaar; Arn M.J.M. van den Maagdenberg; Pasqualina M. Sarro; Massimo Mastrangeli

doi:10.1109/MEMS51670.2022.9699597

Recording Neuronal Activity On Chip with Segmented 3D Microelectrode Arrays

Nele Revyn, Michel H.Y. Hu, Jean-Philippe Frimat, Bjorn de Wagenaar, Arn M.J.M. van den Maagdenberg, Pasqualina M. Sarro, Massimo Mastrangeli

Electronic Components, Technology and Materials

Research output: Chapter in Book/Conference proceedings/Edited volume › Conference contribution › Scientific › peer-review

2 Citations (Scopus)

53 Downloads (Pure)

Abstract

We present preliminary recordings on chip of three-dimensional (3D) electric neuronal activity from cultures of cortical neurons derived from human-induced pluripotent stem cells (hiPSCs). The recordings were obtained through 3D microelectrode arrays (MEAs) composed of truncated, 90 μm-high Si micropyramids endowed with multiple, electrically distinct, and vertically arranged TiN microelectrodes. The unique design and implementation of the 3D microelectrodes, complemented by a 60-electrode readout interface, allow for 3D spatial recording of neuronal activity, as well as single-unit recordings in high throughput, which are currently not possible with commercial MEA platforms. Future work will aim at optimizing extended 3D MEAs over optically transparent substrates for electro-physiological investigation of 3D neuronal tissues and organoids.

Original language	English
Title of host publication	Proceedings of the 2022 IEEE 35th International Conference on Micro Electro Mechanical Systems Conference (MEMS)
Place of Publication	Danvers
Publisher	IEEE
Pages	102-105
Number of pages	4
ISBN (Electronic)	978-1-6654-0911-7
ISBN (Print)	978-1-6654-0912-4
DOIs	https://doi.org/10.1109/MEMS51670.2022.9699597
Publication status	Published - 2022
Event	2022 IEEE 35th International Conference on Micro Electro Mechanical Systems Conference (MEMS) - Hybrid at Tokyo, Japan Duration: 9 Jan 2022 → 13 Jan 2022 Conference number: 35th

Conference

Conference	2022 IEEE 35th International Conference on Micro Electro Mechanical Systems Conference (MEMS)
Abbreviated title	MEMS 2022
Country/Territory	Japan
City	Hybrid at Tokyo
Period	9/01/22 → 13/01/22

Bibliographical note

Green Open Access added to TU Delft Institutional Repository 'You share, we take care!' - Taverne project https://www.openaccess.nl/en/you-share-we-take-care

Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.

Keywords

brain-on-chip
electrodes
electrophysiology
microelectrode array
microfabrication
neurons
organ-on-chip

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10.1109/MEMS51670.2022.9699597

Recording_Neuronal_Activity_On_Chip_with_Segmented_3D_Microelectrode_ArraysFinal published version, 1.31 MB

Cite this

Revyn, N., Hu, M. H. Y., Frimat, J.-P., de Wagenaar, B., van den Maagdenberg, A. M. J. M., Sarro, P. M., & Mastrangeli, M. (2022). Recording Neuronal Activity On Chip with Segmented 3D Microelectrode Arrays. In Proceedings of the 2022 IEEE 35th International Conference on Micro Electro Mechanical Systems Conference (MEMS) (pp. 102-105). Article 9699597 IEEE. https://doi.org/10.1109/MEMS51670.2022.9699597

@inproceedings{98dfd00bdfa64b5fa947d6cee78e3473,

title = "Recording Neuronal Activity On Chip with Segmented 3D Microelectrode Arrays",

abstract = "We present preliminary recordings on chip of three-dimensional (3D) electric neuronal activity from cultures of cortical neurons derived from human-induced pluripotent stem cells (hiPSCs). The recordings were obtained through 3D microelectrode arrays (MEAs) composed of truncated, 90 μm-high Si micropyramids endowed with multiple, electrically distinct, and vertically arranged TiN microelectrodes. The unique design and implementation of the 3D microelectrodes, complemented by a 60-electrode readout interface, allow for 3D spatial recording of neuronal activity, as well as single-unit recordings in high throughput, which are currently not possible with commercial MEA platforms. Future work will aim at optimizing extended 3D MEAs over optically transparent substrates for electro-physiological investigation of 3D neuronal tissues and organoids.",

keywords = "brain-on-chip, electrodes, electrophysiology, microelectrode array, microfabrication, neurons, organ-on-chip",

author = "Nele Revyn and Hu, {Michel H.Y.} and Jean-Philippe Frimat and {de Wagenaar}, Bjorn and {van den Maagdenberg}, {Arn M.J.M.} and Sarro, {Pasqualina M.} and Massimo Mastrangeli",

note = "Green Open Access added to TU Delft Institutional Repository 'You share, we take care!' - Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.; 2022 IEEE 35th International Conference on Micro Electro Mechanical Systems Conference (MEMS), MEMS 2022 ; Conference date: 09-01-2022 Through 13-01-2022",

year = "2022",

doi = "10.1109/MEMS51670.2022.9699597",

language = "English",

isbn = "978-1-6654-0912-4",

pages = "102--105",

booktitle = "Proceedings of the 2022 IEEE 35th International Conference on Micro Electro Mechanical Systems Conference (MEMS)",

publisher = "IEEE",

address = "United States",

}

Revyn, N, Hu, MHY, Frimat, J-P, de Wagenaar, B, van den Maagdenberg, AMJM, Sarro, PM & Mastrangeli, M 2022, Recording Neuronal Activity On Chip with Segmented 3D Microelectrode Arrays. in Proceedings of the 2022 IEEE 35th International Conference on Micro Electro Mechanical Systems Conference (MEMS)., 9699597, IEEE, Danvers, pp. 102-105, 2022 IEEE 35th International Conference on Micro Electro Mechanical Systems Conference (MEMS), Hybrid at Tokyo, Japan, 9/01/22. https://doi.org/10.1109/MEMS51670.2022.9699597

Recording Neuronal Activity On Chip with Segmented 3D Microelectrode Arrays. / Revyn, Nele; Hu, Michel H.Y.; Frimat, Jean-Philippe et al.
Proceedings of the 2022 IEEE 35th International Conference on Micro Electro Mechanical Systems Conference (MEMS). Danvers: IEEE, 2022. p. 102-105 9699597.

Research output: Chapter in Book/Conference proceedings/Edited volume › Conference contribution › Scientific › peer-review

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AU - Revyn, Nele

AU - Hu, Michel H.Y.

AU - Frimat, Jean-Philippe

AU - de Wagenaar, Bjorn

AU - van den Maagdenberg, Arn M.J.M.

AU - Sarro, Pasqualina M.

AU - Mastrangeli, Massimo

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N2 - We present preliminary recordings on chip of three-dimensional (3D) electric neuronal activity from cultures of cortical neurons derived from human-induced pluripotent stem cells (hiPSCs). The recordings were obtained through 3D microelectrode arrays (MEAs) composed of truncated, 90 μm-high Si micropyramids endowed with multiple, electrically distinct, and vertically arranged TiN microelectrodes. The unique design and implementation of the 3D microelectrodes, complemented by a 60-electrode readout interface, allow for 3D spatial recording of neuronal activity, as well as single-unit recordings in high throughput, which are currently not possible with commercial MEA platforms. Future work will aim at optimizing extended 3D MEAs over optically transparent substrates for electro-physiological investigation of 3D neuronal tissues and organoids.

AB - We present preliminary recordings on chip of three-dimensional (3D) electric neuronal activity from cultures of cortical neurons derived from human-induced pluripotent stem cells (hiPSCs). The recordings were obtained through 3D microelectrode arrays (MEAs) composed of truncated, 90 μm-high Si micropyramids endowed with multiple, electrically distinct, and vertically arranged TiN microelectrodes. The unique design and implementation of the 3D microelectrodes, complemented by a 60-electrode readout interface, allow for 3D spatial recording of neuronal activity, as well as single-unit recordings in high throughput, which are currently not possible with commercial MEA platforms. Future work will aim at optimizing extended 3D MEAs over optically transparent substrates for electro-physiological investigation of 3D neuronal tissues and organoids.

KW - brain-on-chip

KW - electrodes

KW - electrophysiology

KW - microelectrode array

KW - microfabrication

KW - neurons

KW - organ-on-chip

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DO - 10.1109/MEMS51670.2022.9699597

M3 - Conference contribution

SN - 978-1-6654-0912-4

SP - 102

EP - 105

BT - Proceedings of the 2022 IEEE 35th International Conference on Micro Electro Mechanical Systems Conference (MEMS)

PB - IEEE

CY - Danvers

T2 - 2022 IEEE 35th International Conference on Micro Electro Mechanical Systems Conference (MEMS)

Y2 - 9 January 2022 through 13 January 2022

ER -

Recording Neuronal Activity On Chip with Segmented 3D Microelectrode Arrays

Abstract

Conference

Bibliographical note

Keywords

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