Reduced acute myocardial ischemia–reperfusion injury in IL-6-deficient mice employing a closed-chest model

Willeke M.C. Jong, Hugo ten Cate, André C. Linnenbank, Onno J. de Boer, Pieter H. Reitsma, Robbert J. de Winter, Coert Zuurbier

Research output: Contribution to journalArticleScientificpeer-review

31 Citations (Scopus)
12 Downloads (Pure)

Abstract

Objective and design: We examined the role of IL-6 in the temporal development of cardiac ischemia–reperfusion injury employing a closed-chest I/R model. Materials/methods: Infarction, local and systemic inflammation, neutrophil infiltration, coagulation and ST elevation/resolution were compared between wild-type (WT) and IL-6-deficient (IL-6−/−) mice after 1 h ischemia and 0, ½, 3, and 24 h reperfusion. Results: IL-6 deficiency reduced infarct size at 3 h reperfusion (28.8 ± 4.5 % WT vs 17.6 ± 2.5 % IL-6−/−), which reduction persisted and remained similar at 24 h reperfusion (25.1 ± 3.0 % WT vs 14.6 ± 4.4 % IL-6−/−). Serum Amyloid A was reduced at 24 h reperfusion only (57.5 ± 4.9 WT vs 24.8 ± 5.6 ug/ml IL-6−/− mice). Cardiac cytokines (IL-6, IL-1β and TNFα) peaked at 3 h reperfusion, but IL-1β and TNFα levels were unaffected by IL-6 deficiency. Significant neutrophil influx was only detected at 24 h reperfusion and was similar for WT and IL-6−/−. Tissue factor peaked at 24 h reperfusion, whereas fibrin/fibrinogen peaked at 3 h reperfusion and was completely resolved at 24 h reperfusion; both coagulation factors were unaltered by IL-6 deficiency. Prolonged ST elevation was observed during ischemia that completely resolved for both genotypes at early reperfusion. Conclusions: The data suggest that, in the absence of major surgical intervention, IL-6 contributes to the development of infarct size in the early phase of reperfusion; this contribution did not depend on neutrophil influx, IL-1β and TNFα, tissue factor and fibrin.

Original languageEnglish
Pages (from-to)489-499
Number of pages11
JournalInflammation Research
Volume65
Issue number6
DOIs
Publication statusPublished - 2016

Keywords

  • Closed-chest model
  • Heart
  • IL-6
  • Inflammation
  • Ischemia/reperfusion injury

Fingerprint

Dive into the research topics of 'Reduced acute myocardial ischemia–reperfusion injury in IL-6-deficient mice employing a closed-chest model'. Together they form a unique fingerprint.

Cite this