Resolution in super-resolution microscopy — definition, trade-offs and perspectives

Kirti Prakash; David Baddeley; Christian Eggeling; Reto Fiolka; Rainer Heintzmann; Suliana Manley; Aleksandra Radenovic; Carlas Smith; Hari Shroff; Lothar Schermelleh

doi:10.1038/s41580-024-00755-7

Resolution in super-resolution microscopy — definition, trade-offs and perspectives

Kirti Prakash^*, David Baddeley^*, Christian Eggeling^*, Reto Fiolka^*, Rainer Heintzmann^*, Suliana Manley^*, Aleksandra Radenovic^*, Carlas Smith^*, Hari Shroff^*, Lothar Schermelleh^*

^*Corresponding author for this work

Team Carlas Smith

Research output: Contribution to journal › Article › Scientific › peer-review

14 Citations (SciVal)

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Abstract

Super-resolution microscopy (SRM) is gaining popularity in biosciences; however, claims about optical resolution are contested and often misleading. In this Viewpoint, experts share their views on resolution and common trade-offs, such as labelling and post-processing, aiming to clarify them for biologists and facilitate deeper understanding and best use of SRM.

Original language	English
Pages (from-to)	677-682
Number of pages	6
Journal	Nature Reviews Molecular Cell Biology
Volume	25
Issue number	9
DOIs	https://doi.org/10.1038/s41580-024-00755-7
Publication status	Published - 2024

Bibliographical note

Green Open Access added to TU Delft Institutional Repository 'You share, we take care!' - Taverne project https://www.openaccess.nl/en/you-share-we-take-care
Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.

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title = "Resolution in super-resolution microscopy — definition, trade-offs and perspectives",

abstract = "Super-resolution microscopy (SRM) is gaining popularity in biosciences; however, claims about optical resolution are contested and often misleading. In this Viewpoint, experts share their views on resolution and common trade-offs, such as labelling and post-processing, aiming to clarify them for biologists and facilitate deeper understanding and best use of SRM.",

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note = "Green Open Access added to TU Delft Institutional Repository 'You share, we take care!' - Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.",

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doi = "10.1038/s41580-024-00755-7",

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AU - Prakash, Kirti

AU - Baddeley, David

AU - Eggeling, Christian

AU - Fiolka, Reto

AU - Heintzmann, Rainer

AU - Manley, Suliana

AU - Radenovic, Aleksandra

AU - Smith, Carlas

AU - Shroff, Hari

AU - Schermelleh, Lothar

N1 - Green Open Access added to TU Delft Institutional Repository 'You share, we take care!' - Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.

PY - 2024

Y1 - 2024

N2 - Super-resolution microscopy (SRM) is gaining popularity in biosciences; however, claims about optical resolution are contested and often misleading. In this Viewpoint, experts share their views on resolution and common trade-offs, such as labelling and post-processing, aiming to clarify them for biologists and facilitate deeper understanding and best use of SRM.

AB - Super-resolution microscopy (SRM) is gaining popularity in biosciences; however, claims about optical resolution are contested and often misleading. In this Viewpoint, experts share their views on resolution and common trade-offs, such as labelling and post-processing, aiming to clarify them for biologists and facilitate deeper understanding and best use of SRM.

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SP - 677

EP - 682

JO - Nature Reviews Molecular Cell Biology

JF - Nature Reviews Molecular Cell Biology

IS - 9

ER -

Resolution in super-resolution microscopy — definition, trade-offs and perspectives

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