TY - JOUR
T1 - Resolving the Complexity of Spatial Lipidomics Using MALDI TIMS Imaging Mass Spectrometry
AU - Djambazova, Katerina V.
AU - Klein, Dustin R.
AU - Migas, Lukasz G.
AU - Neumann, Elizabeth K.
AU - Rivera, Emilio S.
AU - Van De Plas, Raf
AU - Caprioli, Richard M.
AU - Spraggins, Jeffrey M.
PY - 2020
Y1 - 2020
N2 - Lipids are a structurally diverse class of molecules with important biological functions including cellular signaling and energy storage. Matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) allows for direct mapping of biomolecules in tissues. Fully characterizing the structural diversity of lipids remains a challenge due to the presence of isobaric and isomeric species, which greatly complicates data interpretation when only m/z information is available. Integrating ion mobility separations aids in deconvoluting these complex mixtures and addressing the challenges of lipid IMS. Here, we demonstrate that a MALDI quadrupole time-of-flight (Q-TOF) mass spectrometer with trapped ion mobility spectrometry (TIMS) enables a >250% increase in the peak capacity during IMS experiments. MALDI TIMS-MS separation of lipid isomer standards, including sn backbone isomers, acyl chain isomers, and double-bond position and stereoisomers, is demonstrated. As a proof of concept, in situ separation and imaging of lipid isomers with distinct spatial distributions were performed using tissue sections from a whole-body mouse pup.
AB - Lipids are a structurally diverse class of molecules with important biological functions including cellular signaling and energy storage. Matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) allows for direct mapping of biomolecules in tissues. Fully characterizing the structural diversity of lipids remains a challenge due to the presence of isobaric and isomeric species, which greatly complicates data interpretation when only m/z information is available. Integrating ion mobility separations aids in deconvoluting these complex mixtures and addressing the challenges of lipid IMS. Here, we demonstrate that a MALDI quadrupole time-of-flight (Q-TOF) mass spectrometer with trapped ion mobility spectrometry (TIMS) enables a >250% increase in the peak capacity during IMS experiments. MALDI TIMS-MS separation of lipid isomer standards, including sn backbone isomers, acyl chain isomers, and double-bond position and stereoisomers, is demonstrated. As a proof of concept, in situ separation and imaging of lipid isomers with distinct spatial distributions were performed using tissue sections from a whole-body mouse pup.
UR - http://www.scopus.com/inward/record.url?scp=85095974250&partnerID=8YFLogxK
U2 - 10.1021/acs.analchem.0c02520
DO - 10.1021/acs.analchem.0c02520
M3 - Article
AN - SCOPUS:85095974250
SN - 0003-2700
VL - 92
SP - 13290
EP - 13297
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 19
ER -
