Reversible Immobilization of Proteins in Sensors and Solid-State Nanopores

Adithya Ananth, María Genua, Nesrine Aissaoui, Leire Díaz, Nico B. Eisele, Steffen Frey, Cees Dekker, Ralf P. Richter, Dirk Görlich

Research output: Contribution to journalArticleScientificpeer-review

16 Citations (Scopus)
5 Downloads (Pure)

Abstract

The controlled functionalization of surfaces with proteins is crucial for many analytical methods in life science research and biomedical applications. Here, a coating for silica-based surfaces is established which enables stable and selective immobilization of proteins with controlled orientation and tunable surface density. The coating is reusable, retains functionality upon long-term storage in air, and is applicable to surfaces of complex geometry. The protein anchoring method is validated on planar surfaces, and then a method is developed to measure the anchoring process in real time using silicon nitride solid-state nanopores. For surface attachment, polyhistidine tags that are site specifically introduced into recombinant proteins are exploited, and the yeast nucleoporin Nsp1 is used as model protein. Contrary to the commonly used covalent thiol chemistry, the anchoring of proteins via polyhistidine tag is reversible, permitting to take proteins off and replace them by other ones. Such switching in real time in experiments on individual nanopores is monitored using ion conductivity. Finally, it is demonstrated that silica and gold surfaces can be orthogonally functionalized to accommodate polyhistidine-tagged proteins on silica but prevent protein binding to gold, which extends the applicability of this surface functionalization method to even more complex sensor devices.

Original languageEnglish
Article number1703357
JournalSmall
Volume14
Issue number18
DOIs
Publication statusPublished - 3 May 2018

Bibliographical note

Accepted Author Manuscript

Keywords

  • biosensing
  • histidine tag
  • nanopores
  • selective immobilization
  • sensors
  • surface functionalization

Fingerprint

Dive into the research topics of 'Reversible Immobilization of Proteins in Sensors and Solid-State Nanopores'. Together they form a unique fingerprint.

Cite this