Reversibly Triggered Protein-Ligand Assemblies in Giant Vesicles

Ruud J.R.W. Peters, Marlies Nijemeisland, Jan C.M. Van Hest*

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

19 Citations (Scopus)


External small-molecule triggers were used to reversibly control dynamic protein-ligand interactions in giant vesicles. An alcohol dehydrogenase was employed to increase or decrease the interior pH upon conversion of two different small-molecule substrates, thereby modulating the pH-sensitive interaction between a Ni-NTA ligand on the vesicle membrane and an oligohistidine-tagged protein in the lumen. By alternating the small-molecule substrates the interaction could be reversed.

Original languageEnglish
Pages (from-to)9614-9617
Number of pages4
JournalAngewandte Chemie - International Edition
Issue number33
Publication statusPublished - 1 Aug 2015
Externally publishedYes


  • cell mimic
  • liposomes
  • protein-ligand interactions
  • vesicles


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