Rhodococcus strains as source for ene-reductase activity

Bi Shuang Chen, Rosario Médici, Michelle P. van der Helm, Ymke van Zwet, Lorina Gjonaj, Roelien van der Geest, Linda G. Otten, Ulf Hanefeld

Research output: Contribution to journalArticleScientificpeer-review

5 Citations (Scopus)
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Abstract

Rhodococcus strains are ubiquitous in nature and known to metabolise a wide variety of compounds. At the same time, asymmetric reduction of C=C bonds is important in the production of high-valued chiral building blocks. In order to evaluate if Rhodococci can be used for this task, we have probed several Rhodococcus rhodochrous and R. erythropolis strains for ene-reductase activity. A series of substrates including activated ketones, an aldehyde, an imide and nitro-compound were screened using whole cells of seven Rhodococcus strains. This revealed that whole cells of all Rhodococcus strains showed apparent (S)-selectivity towards ketoisophorone, while most other organisms show (R)-selectivity for this compound. Three putative ene-reductases from R. rhodochrous ATCC 17895 were heterologously expressed in Escherichia coli. One protein was purified and its biocatalytic and biochemical properties were characterised, showing typical (enantioselective) properties for class 3 ene-reductases of the old yellow enzyme family.

Original languageEnglish
Pages (from-to)1-12
Number of pages12
JournalApplied Microbiology and Biotechnology
DOIs
Publication statusPublished - 2018

Keywords

  • Asymmetric reduction
  • Enantioselectivity
  • Ene-reductase
  • Rhodococcus

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