TY - JOUR
T1 - Selection and subsequent physiological characterization of industrial Saccharomyces cerevisiae strains during continuous growth at sub- and- supra optimal temperatures
AU - Lip, Ka Ying Florence
AU - García-Ríos, Estéfani
AU - Costa, Carlos E.
AU - Guillamón, José Manuel
AU - Domingues, Lucília
AU - Teixeira, José
AU - van Gulik, Walter M.
PY - 2020
Y1 - 2020
N2 - A phenotypic screening of 12 industrial yeast strains and the well-studied laboratory strain CEN.PK113-7D at cultivation temperatures between 12 °C and 40 °C revealed significant differences in maximum growth rates and temperature tolerance. From those 12, two strains, one performing best at 12 °C and the other at 40 °C, plus the laboratory strain, were selected for further physiological characterization in well-controlled bioreactors. The strains were grown in anaerobic chemostats, at a fixed specific growth rate of 0.03 h−1 and sequential batch cultures at 12 °C, 30 °C, and 39 °C. We observed significant differences in biomass and ethanol yields on glucose, biomass protein and storage carbohydrate contents, and biomass yields on ATP between strains and cultivation temperatures. Increased temperature tolerance coincided with higher energetic efficiency of cell growth, indicating that temperature intolerance is a result of energy wasting processes, such as increased turnover of cellular components (e.g. proteins) due to temperature induced damage.
AB - A phenotypic screening of 12 industrial yeast strains and the well-studied laboratory strain CEN.PK113-7D at cultivation temperatures between 12 °C and 40 °C revealed significant differences in maximum growth rates and temperature tolerance. From those 12, two strains, one performing best at 12 °C and the other at 40 °C, plus the laboratory strain, were selected for further physiological characterization in well-controlled bioreactors. The strains were grown in anaerobic chemostats, at a fixed specific growth rate of 0.03 h−1 and sequential batch cultures at 12 °C, 30 °C, and 39 °C. We observed significant differences in biomass and ethanol yields on glucose, biomass protein and storage carbohydrate contents, and biomass yields on ATP between strains and cultivation temperatures. Increased temperature tolerance coincided with higher energetic efficiency of cell growth, indicating that temperature intolerance is a result of energy wasting processes, such as increased turnover of cellular components (e.g. proteins) due to temperature induced damage.
KW - Chemostat
KW - Energetic efficiency
KW - Saccharomyces
KW - SBR
KW - Temperature tolerance
UR - http://www.scopus.com/inward/record.url?scp=85085027465&partnerID=8YFLogxK
U2 - 10.1016/j.btre.2020.e00462
DO - 10.1016/j.btre.2020.e00462
M3 - Article
AN - SCOPUS:85085027465
SN - 2215-017X
VL - 26
JO - Biotechnology Reports
JF - Biotechnology Reports
M1 - e00462
ER -