SiFLIM: Single-image frequency-domain FLIM provides fast and photon-efficient lifetime data

Marcel Raspe; Katarzyna M. Kedziora; Bram Van Den Broek; Qiaole Zhao; Sander De Jong; Johan Herz; Marieke Mastop; Joachim Goedhart; Theodorus W.J. Gadella; Ian T. Young; Kees Jalink

doi:10.1038/nmeth.3836

SiFLIM: Single-image frequency-domain FLIM provides fast and photon-efficient lifetime data

Marcel Raspe, Katarzyna M. Kedziora, Bram Van Den Broek, Qiaole Zhao, Sander De Jong, Johan Herz, Marieke Mastop, Joachim Goedhart, Theodorus W.J. Gadella, Ian T. Young, Kees Jalink^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Scientific › peer-review

41 Citations (Scopus)

Abstract

We developed single-image fluorescence lifetime imaging microscopy (siFLIM), a method for acquiring quantitative lifetime images from a single exposure. siFLIM takes advantage of a new generation of dedicated cameras that simultaneously record two 180°-phase-shifted images, and it allows for video-rate lifetime imaging with minimal phototoxicity and bleaching. siFLIM is also inherently immune to artifacts stemming from rapid cellular movements and signal transients.

Original language	English
Pages (from-to)	501-504
Number of pages	4
Journal	Nature Methods (online)
Volume	13
Issue number	6
DOIs	https://doi.org/10.1038/nmeth.3836
Publication status	Published - 2016

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10.1038/nmeth.3836

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title = "SiFLIM: Single-image frequency-domain FLIM provides fast and photon-efficient lifetime data",

abstract = "We developed single-image fluorescence lifetime imaging microscopy (siFLIM), a method for acquiring quantitative lifetime images from a single exposure. siFLIM takes advantage of a new generation of dedicated cameras that simultaneously record two 180°-phase-shifted images, and it allows for video-rate lifetime imaging with minimal phototoxicity and bleaching. siFLIM is also inherently immune to artifacts stemming from rapid cellular movements and signal transients.",

author = "Marcel Raspe and Kedziora, {Katarzyna M.} and {Van Den Broek}, Bram and Qiaole Zhao and {De Jong}, Sander and Johan Herz and Marieke Mastop and Joachim Goedhart and Gadella, {Theodorus W.J.} and Young, {Ian T.} and Kees Jalink",

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AU - Raspe, Marcel

AU - Kedziora, Katarzyna M.

AU - Van Den Broek, Bram

AU - Zhao, Qiaole

AU - De Jong, Sander

AU - Herz, Johan

AU - Mastop, Marieke

AU - Goedhart, Joachim

AU - Gadella, Theodorus W.J.

AU - Young, Ian T.

AU - Jalink, Kees

PY - 2016

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AB - We developed single-image fluorescence lifetime imaging microscopy (siFLIM), a method for acquiring quantitative lifetime images from a single exposure. siFLIM takes advantage of a new generation of dedicated cameras that simultaneously record two 180°-phase-shifted images, and it allows for video-rate lifetime imaging with minimal phototoxicity and bleaching. siFLIM is also inherently immune to artifacts stemming from rapid cellular movements and signal transients.

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JF - Nature Methods (online)

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ER -

SiFLIM: Single-image frequency-domain FLIM provides fast and photon-efficient lifetime data

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