SiFLIM: Single-image frequency-domain FLIM provides fast and photon-efficient lifetime data

Marcel Raspe, Katarzyna M. Kedziora, Bram Van Den Broek, Qiaole Zhao, Sander De Jong, Johan Herz, Marieke Mastop, Joachim Goedhart, Theodorus W.J. Gadella, Ian T. Young, Kees Jalink

    Research output: Contribution to journalArticleScientificpeer-review

    28 Citations (Scopus)

    Abstract

    We developed single-image fluorescence lifetime imaging microscopy (siFLIM), a method for acquiring quantitative lifetime images from a single exposure. siFLIM takes advantage of a new generation of dedicated cameras that simultaneously record two 180°-phase-shifted images, and it allows for video-rate lifetime imaging with minimal phototoxicity and bleaching. siFLIM is also inherently immune to artifacts stemming from rapid cellular movements and signal transients.

    Original languageEnglish
    Pages (from-to)501-504
    Number of pages4
    JournalNature Methods (online)
    Volume13
    Issue number6
    DOIs
    Publication statusPublished - 2016

    Fingerprint

    Dive into the research topics of 'SiFLIM: Single-image frequency-domain FLIM provides fast and photon-efficient lifetime data'. Together they form a unique fingerprint.

    Cite this