SiFLIM: Single-image frequency-domain FLIM provides fast and photon-efficient lifetime data

Marcel Raspe, Katarzyna M. Kedziora, Bram Van Den Broek, Qiaole Zhao, Sander De Jong, Johan Herz, Marieke Mastop, Joachim Goedhart, Theodorus W.J. Gadella, Ian T. Young, Kees Jalink

Research output: Contribution to journalArticleScientificpeer-review

22 Citations (Scopus)

Abstract

We developed single-image fluorescence lifetime imaging microscopy (siFLIM), a method for acquiring quantitative lifetime images from a single exposure. siFLIM takes advantage of a new generation of dedicated cameras that simultaneously record two 180°-phase-shifted images, and it allows for video-rate lifetime imaging with minimal phototoxicity and bleaching. siFLIM is also inherently immune to artifacts stemming from rapid cellular movements and signal transients.

Original languageEnglish
Pages (from-to)501-504
Number of pages4
JournalNature Methods (online)
Volume13
Issue number6
DOIs
Publication statusPublished - 2016

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    Raspe, M., Kedziora, K. M., Van Den Broek, B., Zhao, Q., De Jong, S., Herz, J., Mastop, M., Goedhart, J., Gadella, T. W. J., Young, I. T., & Jalink, K. (2016). SiFLIM: Single-image frequency-domain FLIM provides fast and photon-efficient lifetime data. Nature Methods (online), 13(6), 501-504. https://doi.org/10.1038/nmeth.3836