TY - JOUR
T1 - Simultaneous nitrification and phosphate removal by bioaugmented aerobic granules treating a fluoroorganic compound
AU - Duque, Anouk F.
AU - Bessa, Vânia S.
AU - van Dongen, Udo
AU - de Kreuk, Merle K.
AU - Mesquita, Raquel B.R.
AU - Rangel, António O.S.S.
AU - van Loosdrecht, Mark C.M.
AU - Castro, Paula M.L.
PY - 2021
Y1 - 2021
N2 - The presence of toxic compounds in wastewater can cause problems for organic matter and nutrient removal. In this study, the long-term effect of a model xenobiotic, 2-fluorophenol (2-FP), on ammonia-oxidizing bacteria (AOB), nitrite oxidizing bacteria (NOB) and phosphate accumulating organisms (PAO) in aerobic granular sludge was investigated. Phosphate (P) and ammonium (N) removal efficiencies were high (>93%) and, after bioaugmentation with 2-FP degrading strain FP1, 2-FP was completely degraded. Neither N nor P removal were affected by 50 mg L1 of 2-FP in the feed stream. Changes in the aerobic granule bacterial communities were followed. Numerical analysis of the denaturing gradient gel electrophoresis (DGGE) profiles showed low diversity for the ammonia monooxygenase (amoA) gene with an even distribution of species. PAOs, including denitrifying PAO (dPAO), and AOB were present in the 2-FP degrading granules, although dPAO population decreased throughout the 444 days reactor operation. The results demonstrated that the aerobic granules bioaugmented with FP1 strain successfully removed N, P and 2-FP simultaneously.
AB - The presence of toxic compounds in wastewater can cause problems for organic matter and nutrient removal. In this study, the long-term effect of a model xenobiotic, 2-fluorophenol (2-FP), on ammonia-oxidizing bacteria (AOB), nitrite oxidizing bacteria (NOB) and phosphate accumulating organisms (PAO) in aerobic granular sludge was investigated. Phosphate (P) and ammonium (N) removal efficiencies were high (>93%) and, after bioaugmentation with 2-FP degrading strain FP1, 2-FP was completely degraded. Neither N nor P removal were affected by 50 mg L1 of 2-FP in the feed stream. Changes in the aerobic granule bacterial communities were followed. Numerical analysis of the denaturing gradient gel electrophoresis (DGGE) profiles showed low diversity for the ammonia monooxygenase (amoA) gene with an even distribution of species. PAOs, including denitrifying PAO (dPAO), and AOB were present in the 2-FP degrading granules, although dPAO population decreased throughout the 444 days reactor operation. The results demonstrated that the aerobic granules bioaugmented with FP1 strain successfully removed N, P and 2-FP simultaneously.
KW - 2-fluorophenol (2-FP)
KW - Aerobic granular sludge
KW - Ammonia-oxidizing bacteria (AOB)
KW - Microbial population dynamics
KW - Nitrite oxidizing bacteria (NOB)
KW - Phosphate accumulating organisms (PAO)
UR - http://www.scopus.com/inward/record.url?scp=85106909949&partnerID=8YFLogxK
U2 - 10.2166/wst.2021.142
DO - 10.2166/wst.2021.142
M3 - Article
C2 - 34032618
AN - SCOPUS:85106909949
SN - 0273-1223
VL - 83
SP - 2404
EP - 2413
JO - Water Science and Technology
JF - Water Science and Technology
IS - 10
ER -