Single-Molecule FRET X

Mike Filius; Raman van Wee; Chirlmin Joo

doi:10.1007/978-1-0716-3377-9_10

Single-Molecule FRET X

Mike Filius, Raman van Wee, Chirlmin Joo^*

^*Corresponding author for this work

BN/Chirlmin Joo Lab

Research output: Chapter in Book/Conference proceedings/Edited volume › Chapter › Scientific › peer-review

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Abstract

Fluorescence resonance energy transfer (FRET) is a photophysical phenomenon that has been repurposed as a biophysical tool to measure nanometer distances. With FRET by DNA eXchange, or FRET X, many points of interest (POIs) in a single object can be probed, overcoming a major limitation of conventional single-molecule FRET. In FRET X, short fluorescently labeled DNA imager strands specifically and transiently bind their complementary docking strands on a target molecule, such that at most a single FRET pair is formed at each point in time and multiple POIs on a single molecule can be readily probed. Here, we describe the sample preparation, image acquisition, and data analysis for structural analysis of DNA nanostructures with FRET X.

Original language	English
Title of host publication	Single Molecule Analysis
Editors	Iddo Heller, David Dulin, Erwin J.G. Peterman
Publisher	Humana Press Inc.
Pages	203-213
Number of pages	11
ISBN (Electronic)	978-1-0716-3377-9
ISBN (Print)	978-1-0716-3379-3
DOIs	https://doi.org/10.1007/978-1-0716-3377-9_10
Publication status	Published - 2023

Publication series

Name	Methods in Molecular Biology
Volume	2694
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

Green Open Access added to TU Delft Institutional Repository 'You share, we take care!' - Taverne project https://www.openaccess.nl/en/you-share-we-take-care
Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.

Keywords

DNA nanotechnology
FRET X
Single-molecule FRET
Single-molecule multiplexing
Structural biology

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10.1007/978-1-0716-3377-9_10

978-1-0716-3377-9_10Final published version, 2.71 MB

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title = "Single-Molecule FRET X",

abstract = "Fluorescence resonance energy transfer (FRET) is a photophysical phenomenon that has been repurposed as a biophysical tool to measure nanometer distances. With FRET by DNA eXchange, or FRET X, many points of interest (POIs) in a single object can be probed, overcoming a major limitation of conventional single-molecule FRET. In FRET X, short fluorescently labeled DNA imager strands specifically and transiently bind their complementary docking strands on a target molecule, such that at most a single FRET pair is formed at each point in time and multiple POIs on a single molecule can be readily probed. Here, we describe the sample preparation, image acquisition, and data analysis for structural analysis of DNA nanostructures with FRET X.",

keywords = "DNA nanotechnology, FRET X, Single-molecule FRET, Single-molecule multiplexing, Structural biology",

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note = "Green Open Access added to TU Delft Institutional Repository 'You share, we take care!' - Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.",

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AU - Joo, Chirlmin

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PY - 2023

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N2 - Fluorescence resonance energy transfer (FRET) is a photophysical phenomenon that has been repurposed as a biophysical tool to measure nanometer distances. With FRET by DNA eXchange, or FRET X, many points of interest (POIs) in a single object can be probed, overcoming a major limitation of conventional single-molecule FRET. In FRET X, short fluorescently labeled DNA imager strands specifically and transiently bind their complementary docking strands on a target molecule, such that at most a single FRET pair is formed at each point in time and multiple POIs on a single molecule can be readily probed. Here, we describe the sample preparation, image acquisition, and data analysis for structural analysis of DNA nanostructures with FRET X.

AB - Fluorescence resonance energy transfer (FRET) is a photophysical phenomenon that has been repurposed as a biophysical tool to measure nanometer distances. With FRET by DNA eXchange, or FRET X, many points of interest (POIs) in a single object can be probed, overcoming a major limitation of conventional single-molecule FRET. In FRET X, short fluorescently labeled DNA imager strands specifically and transiently bind their complementary docking strands on a target molecule, such that at most a single FRET pair is formed at each point in time and multiple POIs on a single molecule can be readily probed. Here, we describe the sample preparation, image acquisition, and data analysis for structural analysis of DNA nanostructures with FRET X.

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KW - FRET X

KW - Single-molecule FRET

KW - Single-molecule multiplexing

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A2 - Dulin, David

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PB - Humana Press Inc.

ER -

Single-Molecule FRET X

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