Single objective tilted lightsheet for three-dimensional localization microscopy

Shih Te Hung; Jelmer Cnossen; Daniel Fan; Carlas S. Smith

Single objective tilted lightsheet for three-dimensional localization microscopy

Shih Te Hung, Jelmer Cnossen, Daniel Fan, Carlas S. Smith^*

^*Corresponding author for this work

Team Carlas Smith

Research output: Chapter in Book/Conference proceedings/Edited volume › Conference contribution › Scientific › peer-review

Abstract

Optical sectioning technologies achieve high precision localization by reducing the background photon count. We use tilted light-sheet microscopy to achieve optical sectioning in localization microscopy, enabling thick sample observation and low background photon count images. A deformable mirror was incorporated to generate a tetrapod point spread function (PSF), enabling high resolution 3D localization. DNA-PAINT was imaged with 15 nm transverse and 60 nm axial resolution.

Original language	English
Title of host publication	European Conferences on Biomedical Optics, ECBO 2021
Publisher	OSA - The Optical Society
Number of pages	2
ISBN (Electronic)	978-1-557528-20-9
ISBN (Print)	978-1-943580-95-8
Publication status	Published - 2021
Event	2021 European Conferences on Biomedical Optics, ECBO 2021 - Virtual, Online, Germany Duration: 20 Jun 2021 → 24 Jun 2021

Conference

Conference	2021 European Conferences on Biomedical Optics, ECBO 2021
Country/Territory	Germany
City	Virtual, Online
Period	20/06/21 → 24/06/21

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https://www.osapublishing.org/abstract.cfm?uri=ECBO-2021-EM1A.37

Cite this

@inproceedings{63ec8c6b016c47ef9d201df2bb16bc4e,

title = "Single objective tilted lightsheet for three-dimensional localization microscopy",

abstract = "Optical sectioning technologies achieve high precision localization by reducing the background photon count. We use tilted light-sheet microscopy to achieve optical sectioning in localization microscopy, enabling thick sample observation and low background photon count images. A deformable mirror was incorporated to generate a tetrapod point spread function (PSF), enabling high resolution 3D localization. DNA-PAINT was imaged with 15 nm transverse and 60 nm axial resolution.",

author = "Hung, {Shih Te} and Jelmer Cnossen and Daniel Fan and Smith, {Carlas S.}",

year = "2021",

language = "English",

isbn = "978-1-943580-95-8",

booktitle = "European Conferences on Biomedical Optics, ECBO 2021",

publisher = "OSA - The Optical Society",

address = "United States",

note = "2021 European Conferences on Biomedical Optics, ECBO 2021 ; Conference date: 20-06-2021 Through 24-06-2021",

}

Hung, ST , Cnossen, J, Fan, D & Smith, CS 2021, Single objective tilted lightsheet for three-dimensional localization microscopy. in European Conferences on Biomedical Optics, ECBO 2021., EM1A.37, OSA - The Optical Society, 2021 European Conferences on Biomedical Optics, ECBO 2021, Virtual, Online, Germany, 20/06/21. <https://www.osapublishing.org/abstract.cfm?uri=ECBO-2021-EM1A.37>

TY - GEN

T1 - Single objective tilted lightsheet for three-dimensional localization microscopy

AU - Hung, Shih Te

AU - Cnossen, Jelmer

AU - Fan, Daniel

AU - Smith, Carlas S.

PY - 2021

Y1 - 2021

N2 - Optical sectioning technologies achieve high precision localization by reducing the background photon count. We use tilted light-sheet microscopy to achieve optical sectioning in localization microscopy, enabling thick sample observation and low background photon count images. A deformable mirror was incorporated to generate a tetrapod point spread function (PSF), enabling high resolution 3D localization. DNA-PAINT was imaged with 15 nm transverse and 60 nm axial resolution.

AB - Optical sectioning technologies achieve high precision localization by reducing the background photon count. We use tilted light-sheet microscopy to achieve optical sectioning in localization microscopy, enabling thick sample observation and low background photon count images. A deformable mirror was incorporated to generate a tetrapod point spread function (PSF), enabling high resolution 3D localization. DNA-PAINT was imaged with 15 nm transverse and 60 nm axial resolution.

UR - http://www.scopus.com/inward/record.url?scp=85119452080&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:85119452080

SN - 978-1-943580-95-8

BT - European Conferences on Biomedical Optics, ECBO 2021

PB - OSA - The Optical Society

T2 - 2021 European Conferences on Biomedical Optics, ECBO 2021

Y2 - 20 June 2021 through 24 June 2021

ER -

Single objective tilted lightsheet for three-dimensional localization microscopy

Abstract

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