Single objective tilted lightsheet for three-dimensional localization microscopy

Shih Te Hung, Jelmer Cnossen, Daniel Fan, Carlas S. Smith*

*Corresponding author for this work

Research output: Chapter in Book/Conference proceedings/Edited volumeConference contributionScientificpeer-review

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Abstract

Optical sectioning technologies achieve high precision localization by reducing the background photon count. We use tilted light-sheet microscopy to achieve optical sectioning in localization microscopy, enabling thick sample observation and low background photon count images. A deformable mirror was incorporated to generate a tetrapod point spread function (PSF), enabling high resolution 3D localization. DNA-PAINT was imaged with 15 nm transverse and 60 nm axial resolution.

Original languageEnglish
Title of host publicationAdvances in Microscopic Imaging III
Subtitle of host publicationProceedings
EditorsEmmanuel Beaurepaire, Adela Ben-Yakar, YongKeun Park
PublisherSPIE
Number of pages2
ISBN (Electronic)9781510647107
DOIs
Publication statusPublished - 2021
EventAdvances in Microscopic Imaging III 2021 - Virtual, Online, Germany
Duration: 20 Jun 202124 Jun 2021

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume11922
ISSN (Print)1605-7422

Conference

ConferenceAdvances in Microscopic Imaging III 2021
Country/TerritoryGermany
CityVirtual, Online
Period20/06/2124/06/21

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