Solvent-Free Photobiocatalytic Hydroxylation of Cyclohexane

Markus Hobisch, Morten Martinus Cornelis Harald van Schie, Jinhyun Kim, Kasper Røjkjær Andersen, Miguel Alcalde, Robert Kourist, Chan Beum Park, Frank Hollmann, Selin Kara

Research output: Contribution to journalArticleScientificpeer-review

4 Citations (Scopus)
7 Downloads (Pure)


The use of neat reaction media, that is the avoidance of additional solvents, is the simplest and the most efficient approach to follow in biocatalysis. Here, we show that unspecific peroxygenase from Agrocybe aegerita (AaeUPO) can hydroxylate the neat model substrate cyclohexane. H2O2 was photocatalytically generated in situ by nitrogen-doped carbon nanodots (N−CNDs) and UV LED illumination. AaeUPO entrapment in alginate beads increased enzyme stability and facilitated the reaction in neat cyclohexane. N−CNDs absorption in beads containing AaeUPO created a 2-in-1 heterogeneous photobiocatalyst that was active for up to seven days under reaction conditions and produced cyclohexanol, 2.5 mM. To increase productivity, the bead size and the photocatalyst-to-enzyme ratio have been identified as promising targets for optimisation.

Original languageEnglish
Pages (from-to)4009-4013
Number of pages5
Issue number16
Publication statusPublished - 2020


  • carbon nanodots
  • hydroxylation
  • non-conventional media
  • organic media
  • Photobiocatalysis

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