SpaceWalker enables interactive gradient exploration for spatial transcriptomics data

Chang Li, Julian Thijssen, Thomas Kroes, Mitchell de Boer, Tamim Abdelaal, Thomas Höllt, Boudewijn Lelieveldt*

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

In spatial transcriptomics (ST) data, biologically relevant features such as tissue compartments or cell-state transitions are reflected by gene expression gradients. Here, we present SpaceWalker, a visual analytics tool for exploring the local gradient structure of 2D and 3D ST data. The user can be guided by the local intrinsic dimensionality of the high-dimensional data to define seed locations, from which a flood-fill algorithm identifies transcriptomically similar cells on the fly, based on the high-dimensional data topology. In several use cases, we demonstrate that the spatial projection of these flooded cells highlights tissue architectural features and that interactive retrieval of gene expression gradients in the spatial and transcriptomic domains confirms known biology. We also show that SpaceWalker generalizes to several different ST protocols and scales well to large, multi-slice, 3D whole-brain ST data while maintaining real-time interaction performance.

Original languageEnglish
Article number100645
Number of pages15
JournalCell Reports Methods
Volume3
Issue number12
DOIs
Publication statusPublished - 2023

Keywords

  • data visualization
  • visual analytics
  • spatial transcriptomics
  • gene expression gradients

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