Stabilisation of the Fatty Acid Decarboxylase from Chlorella variabilis by Caprylic Acid

Yinqi Wu; Caroline E. Paul; Frank Hollmann

doi:10.1002/cbic.202100182

Stabilisation of the Fatty Acid Decarboxylase from Chlorella variabilis by Caprylic Acid

Yinqi Wu, Caroline E. Paul, Frank Hollmann^*

^*Corresponding author for this work

BT/Biocatalysis

Research output: Contribution to journal › Article › Scientific › peer-review

25 Citations (Scopus)

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Abstract

The fatty acid photodecarboxylase from Chlorella variabilis NC64 A (CvFAP) catalyses the light-dependent decarboxylation of fatty acids. Photoinactivation of CvFAP still represents one of the major limitations of this interesting enzyme en route to practical application. In this study we demonstrate that the photostability of CvFAP can easily be improved by the administration of medium-chain length carboxylic acids such as caprylic acid indicating that the best way of maintaining CvFAP stability is ‘to keep the enzyme busy’.

Original language	English
Pages (from-to)	2420-2423
Number of pages	4
Journal	ChemBioChem
Volume	22
Issue number	14
DOIs	https://doi.org/10.1002/cbic.202100182
Publication status	Published - 2021

Keywords

caprylic acid
fatty acid photodecarboxylase
fatty acids
photochemical inactivation
photostability

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10.1002/cbic.202100182

cbic.202100182Final published version, 2.71 MBLicence: CC BY

Cite this

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title = "Stabilisation of the Fatty Acid Decarboxylase from Chlorella variabilis by Caprylic Acid",

abstract = "The fatty acid photodecarboxylase from Chlorella variabilis NC64 A (CvFAP) catalyses the light-dependent decarboxylation of fatty acids. Photoinactivation of CvFAP still represents one of the major limitations of this interesting enzyme en route to practical application. In this study we demonstrate that the photostability of CvFAP can easily be improved by the administration of medium-chain length carboxylic acids such as caprylic acid indicating that the best way of maintaining CvFAP stability is {\textquoteleft}to keep the enzyme busy{\textquoteright}.",

keywords = "caprylic acid, fatty acid photodecarboxylase, fatty acids, photochemical inactivation, photostability",

author = "Yinqi Wu and Paul, {Caroline E.} and Frank Hollmann",

year = "2021",

doi = "10.1002/cbic.202100182",

language = "English",

volume = "22",

pages = "2420--2423",

journal = "ChemBioChem",

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T1 - Stabilisation of the Fatty Acid Decarboxylase from Chlorella variabilis by Caprylic Acid

AU - Wu, Yinqi

AU - Paul, Caroline E.

AU - Hollmann, Frank

PY - 2021

Y1 - 2021

N2 - The fatty acid photodecarboxylase from Chlorella variabilis NC64 A (CvFAP) catalyses the light-dependent decarboxylation of fatty acids. Photoinactivation of CvFAP still represents one of the major limitations of this interesting enzyme en route to practical application. In this study we demonstrate that the photostability of CvFAP can easily be improved by the administration of medium-chain length carboxylic acids such as caprylic acid indicating that the best way of maintaining CvFAP stability is ‘to keep the enzyme busy’.

AB - The fatty acid photodecarboxylase from Chlorella variabilis NC64 A (CvFAP) catalyses the light-dependent decarboxylation of fatty acids. Photoinactivation of CvFAP still represents one of the major limitations of this interesting enzyme en route to practical application. In this study we demonstrate that the photostability of CvFAP can easily be improved by the administration of medium-chain length carboxylic acids such as caprylic acid indicating that the best way of maintaining CvFAP stability is ‘to keep the enzyme busy’.

KW - caprylic acid

KW - fatty acid photodecarboxylase

KW - fatty acids

KW - photochemical inactivation

KW - photostability

UR - http://www.scopus.com/inward/record.url?scp=85107337282&partnerID=8YFLogxK

U2 - 10.1002/cbic.202100182

DO - 10.1002/cbic.202100182

M3 - Article

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AN - SCOPUS:85107337282

SN - 1439-4227

VL - 22

SP - 2420

EP - 2423

JO - ChemBioChem

JF - ChemBioChem

IS - 14

ER -

Stabilisation of the Fatty Acid Decarboxylase from Chlorella variabilis by Caprylic Acid

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Data underlying the publication: Stabilisation of the fatty acid decarboxylase from Chlorella variabilis by caprylic acid

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Stabilisation of the Fatty Acid Decarboxylase from Chlorella variabilis by Caprylic Acid

Abstract

Keywords

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Data underlying the publication: Stabilisation of the fatty acid decarboxylase from Chlorella variabilis by caprylic acid

Cite this