TY - JOUR
T1 - Stoichiometry and kinetics of single and mixed substrate uptake in Aspergillus niger
AU - Da Fonte Lameiras, F.
AU - Ras, Cor
AU - Ten Pierick, Angela
AU - Heijnen, Joseph J.
AU - van Gulik, Walter M.
PY - 2017/10/19
Y1 - 2017/10/19
N2 - In its natural environment, the filamentous fungus Aspergillus niger grows on decaying fruits and plant material, thereby enzymatically degrading the lignocellulosic constituents (lignin, cellulose, hemicellulose, and pectin) into a mixture of mono- and oligosaccharides. To investigate the kinetics and stoichiometry of growth of this fungus on lignocellulosic sugars, we carried out batch cultivations on six representative monosaccharides (glucose, xylose, mannose, rhamnose, arabinose, and galacturonic acid) and a mixture of these. Growth on these substrates was characterized in terms of biomass yields, oxygen/biomass ratios, and specific conversion rates. Interestingly, in combination, some of the carbon sources were consumed simultaneously and some sequentially. With a previously developed protocol, a sequential chemostat cultivation experiment was performed on a feed mixture of the six substrates. We found that the uptake of glucose, xylose, and mannose could be described with a Michaelis–Menten-type kinetics; however, these carbon sources seem to be competing for the same transport systems, while the uptake of arabinose, galacturonic acid, and rhamnose appeared to be repressed by the presence of other substrates.
AB - In its natural environment, the filamentous fungus Aspergillus niger grows on decaying fruits and plant material, thereby enzymatically degrading the lignocellulosic constituents (lignin, cellulose, hemicellulose, and pectin) into a mixture of mono- and oligosaccharides. To investigate the kinetics and stoichiometry of growth of this fungus on lignocellulosic sugars, we carried out batch cultivations on six representative monosaccharides (glucose, xylose, mannose, rhamnose, arabinose, and galacturonic acid) and a mixture of these. Growth on these substrates was characterized in terms of biomass yields, oxygen/biomass ratios, and specific conversion rates. Interestingly, in combination, some of the carbon sources were consumed simultaneously and some sequentially. With a previously developed protocol, a sequential chemostat cultivation experiment was performed on a feed mixture of the six substrates. We found that the uptake of glucose, xylose, and mannose could be described with a Michaelis–Menten-type kinetics; however, these carbon sources seem to be competing for the same transport systems, while the uptake of arabinose, galacturonic acid, and rhamnose appeared to be repressed by the presence of other substrates.
KW - Aspergillus niger
KW - Kinetics
KW - Plant waste streams
KW - Stoichiometry
KW - Substrate uptake
UR - http://resolver.tudelft.nl/uuid:e2ecf3aa-ea71-41f1-a1c3-a51ca6b4d9ff
UR - http://www.scopus.com/inward/record.url?scp=85031787768&partnerID=8YFLogxK
U2 - 10.1007/s00449-017-1854-3
DO - 10.1007/s00449-017-1854-3
M3 - Article
AN - SCOPUS:85031787768
SN - 1615-7591
SP - 1
EP - 14
JO - Bioprocess and Biosystems Engineering: bioreactors, upstream and downstream processes, measurement and control
JF - Bioprocess and Biosystems Engineering: bioreactors, upstream and downstream processes, measurement and control
ER -