TY - JOUR
T1 - Super-resolution fight club
T2 - Assessment of 2D and 3D single-molecule localization microscopy software
AU - Sage, D
AU - Pham, Thanh-An
AU - Babcock, Hazen
AU - Lukes, Tomas
AU - Pengo, Thomas
AU - Velmurugan, Ramraj
AU - Herbert, Alex
AU - Agrawal, Anurag
AU - Rieger, Bernd
AU - More Authors, null
PY - 2019
Y1 - 2019
N2 - With the widespread uptake of 2D and 3D single molecule localization microscopy, a large set of different data analysis packages have been developed to generate super-resolution images. To guide researchers on the optimal analytical software for their experiments, we have designed, in a large community effort, a competition to extensively characterise and rank these options. We generated realistic simulated datasets for popular imaging modalities - 2D, astigmatic 3D, biplane 3D, and double helix 3D - and evaluated 36 participant packages against these data. This provides the first broad assessment of 3D single molecule localization microscopy software, provides a holistic view of how the latest 2D and 3D single molecule localization software perform in realistic conditions, and ultimately provides insight into the current limits of the field.
AB - With the widespread uptake of 2D and 3D single molecule localization microscopy, a large set of different data analysis packages have been developed to generate super-resolution images. To guide researchers on the optimal analytical software for their experiments, we have designed, in a large community effort, a competition to extensively characterise and rank these options. We generated realistic simulated datasets for popular imaging modalities - 2D, astigmatic 3D, biplane 3D, and double helix 3D - and evaluated 36 participant packages against these data. This provides the first broad assessment of 3D single molecule localization microscopy software, provides a holistic view of how the latest 2D and 3D single molecule localization software perform in realistic conditions, and ultimately provides insight into the current limits of the field.
UR - http://www.scopus.com/inward/record.url?scp=85064068699&partnerID=8YFLogxK
U2 - 10.1038/s41592-019-0364-4
DO - 10.1038/s41592-019-0364-4
M3 - Article
SN - 1548-7105
VL - 16
SP - 387
EP - 395
JO - Nature Methods (online)
JF - Nature Methods (online)
IS - 5
ER -