Super-resolution fight club: Assessment of 2D and 3D single-molecule localization microscopy software

D Sage, Thanh-An Pham, Hazen Babcock, Tomas Lukes, Thomas Pengo, Ramraj Velmurugan, Alex Herbert, Anurag Agrawal, Bernd Rieger, More Authors

    Research output: Contribution to journalArticleScientific

    81 Citations (Scopus)

    Abstract

    With the widespread uptake of 2D and 3D single molecule localization microscopy, a large set of different data analysis packages have been developed to generate super-resolution images. To guide researchers on the optimal analytical software for their experiments, we have designed, in a large community effort, a competition to extensively characterise and rank these options. We generated realistic simulated datasets for popular imaging modalities - 2D, astigmatic 3D, biplane 3D, and double helix 3D - and evaluated 36 participant packages against these data. This provides the first broad assessment of 3D single molecule localization microscopy software, provides a holistic view of how the latest 2D and 3D single molecule localization software perform in realistic conditions, and ultimately provides insight into the current limits of the field.
    Original languageEnglish
    Pages (from-to)387-395
    Number of pages9
    JournalNature Methods (online)
    Volume16
    Issue number5
    DOIs
    Publication statusPublished - 2019

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