Synthesis of novel laccase-biotitania biocatalysts for malachite green decolorization

Xinying Zhang, Meiyin Wang, Linlin Lin, Gao Xiao, Zhenping Tang, Xuefeng Zhu

Research output: Contribution to journalArticleScientificpeer-review

17 Citations (Scopus)

Abstract

Biomimetic mineralization has emerged as a novel tool for generating excellent supports for enzyme stabilization. In this work, protamine was used to induce titanium (IV) bis(ammonium lactato) dihydroxide (Ti-BALDH) into titania nanoparticles. This biomimetic titanification process was adopted for laccase immobilization. Laccase-biotitania biocatalyst was prepared and the effect of different parameters (buffer solution, titania precursor concentration, protamine concentration, and enzyme loading) on the encapsulation efficiency and recovery of laccase were evaluated. Compared with free laccase, the thermal and pH stability of immobilized laccase were improved significantly. In addition, laccase loaded on titania was effective at enhancing its storage stability. After seven consecutive cycles, the immobilized laccase still retained 51% of its original activity. Finally, laccase-biotitania biocatalysts showed good performance on decolorization of malachite green (MG), which can be attributed to an adsorption and degradation effect. The intermediates of the MG degradation were identified by gas chromatography-mass spectrometry (GC–MS) analysis, and the most probable degradation pathway was proposed. This study provides deeper understanding of the laccase-biotitania particles as a fast biocatalyst for MG decolorization.

Original languageEnglish
Pages (from-to)69-77
JournalJournal of Bioscience and Bioengineering
Volume126
Issue number1
DOIs
Publication statusPublished - 2018

Keywords

  • Biomimetic titania
  • Enzyme immobilization
  • Laccase
  • Malachite green
  • Wastewater treatment

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