TY - JOUR
T1 - Synthetic Polymers Provide a Robust Substrate for Functional Neuron Culture
AU - Zhang, Yichuan
AU - Venkateswaran, Seshasailam
AU - Higuera, Gustavo A.
AU - Nath, Suvra
AU - Shpak, Guy
AU - Matray, Jeffrey
AU - Fratila-Apachitei, Lidy E.
AU - Zadpoor, Amir A.
AU - Kushner, Steven A.
AU - Bradley, Mark
AU - De Zeeuw, Chris I.
PY - 2020
Y1 - 2020
N2 - Substrates for neuron culture and implantation are required to be both biocompatible and display surface compositions that support cell attachment, growth, differentiation, and neural activity. Laminin, a naturally occurring extracellular matrix protein is the most widely used substrate for neuron culture and fulfills some of these requirements, however, it is expensive, unstable (compared to synthetic materials), and prone to batch-to-batch variation. This study uses a high-throughput polymer screening approach to identify synthetic polymers that supports the in vitro culture of primary mouse cerebellar neurons. This allows the identification of materials that enable primary cell attachment with high viability even under “serum-free” conditions, with materials that support both primary cells and neural progenitor cell attachment with high levels of neuronal biomarker expression, while promoting progenitor cell maturation to neurons.
AB - Substrates for neuron culture and implantation are required to be both biocompatible and display surface compositions that support cell attachment, growth, differentiation, and neural activity. Laminin, a naturally occurring extracellular matrix protein is the most widely used substrate for neuron culture and fulfills some of these requirements, however, it is expensive, unstable (compared to synthetic materials), and prone to batch-to-batch variation. This study uses a high-throughput polymer screening approach to identify synthetic polymers that supports the in vitro culture of primary mouse cerebellar neurons. This allows the identification of materials that enable primary cell attachment with high viability even under “serum-free” conditions, with materials that support both primary cells and neural progenitor cell attachment with high levels of neuronal biomarker expression, while promoting progenitor cell maturation to neurons.
KW - central nervous system regeneration
KW - neuron cultures
KW - polymer microarrays
KW - progenitor cell maturation
KW - synthetic polymer substrates
UR - http://www.scopus.com/inward/record.url?scp=85078046506&partnerID=8YFLogxK
U2 - 10.1002/adhm.201901347
DO - 10.1002/adhm.201901347
M3 - Article
C2 - 31943855
AN - SCOPUS:85078046506
SN - 2192-2640
VL - 9
JO - Advanced Healthcare Materials
JF - Advanced Healthcare Materials
IS - 4
M1 - 1901347
ER -