Template-free 2D particle fusion in localization microscopy

Hamidreza Heydarian, Florian Schueder, Maximilian T. Strauss, Ben van Werkhoven, Mohamadreza Fazel, Keith A. Lidke, Ralf Jungmann, Sjoerd Stallinga, Bernd Rieger

Research output: Contribution to journalArticleScientificpeer-review

9 Citations (Scopus)
69 Downloads (Pure)


Methods that fuse multiple localization microscopy images of a single structure can improve signal-to-noise ratio and resolution, but they generally suffer from template bias or sensitivity to registration errors. We present a template-free particle-fusion approach based on an all-to-all registration that provides robustness against individual misregistrations and underlabeling. We achieved 3.3-nm Fourier ring correlation (FRC) image resolution by fusing 383 DNA origami nanostructures with 80% labeling density, and 5.0-nm resolution for structures with 30% labeling density.

Original languageEnglish
Pages (from-to)781-784
JournalNature Methods
Publication statusPublished - 2018

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  • Research Output

    • 9 Citations
    • 1 Dissertation (TU Delft)

    Particle fusion in localization microscopy

    Heydarian, H., 17 Mar 2020, (In preparation) Delft. 96 p.

    Research output: ThesisDissertation (TU Delft)

  • 70 Downloads (Pure)

    Cite this

    Heydarian, H., Schueder, F., Strauss, M. T., van Werkhoven, B., Fazel, M., Lidke, K. A., Jungmann, R., Stallinga, S., & Rieger, B. (2018). Template-free 2D particle fusion in localization microscopy. Nature Methods, 15, 781-784. https://doi.org/10.1038/s41592-018-0136-6