TY - JOUR
T1 - Template-free 2D particle fusion in localization microscopy
AU - Heydarian, Hamidreza
AU - Schueder, Florian
AU - Strauss, Maximilian T.
AU - van Werkhoven, Ben
AU - Fazel, Mohamadreza
AU - Lidke, Keith A.
AU - Jungmann, Ralf
AU - Stallinga, Sjoerd
AU - Rieger, Bernd
N1 - Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.
PY - 2018
Y1 - 2018
N2 - Methods that fuse multiple localization microscopy images of a single structure can improve signal-to-noise ratio and resolution, but they generally suffer from template bias or sensitivity to registration errors. We present a template-free particle-fusion approach based on an all-to-all registration that provides robustness against individual misregistrations and underlabeling. We achieved 3.3-nm Fourier ring correlation (FRC) image resolution by fusing 383 DNA origami nanostructures with 80% labeling density, and 5.0-nm resolution for structures with 30% labeling density.
AB - Methods that fuse multiple localization microscopy images of a single structure can improve signal-to-noise ratio and resolution, but they generally suffer from template bias or sensitivity to registration errors. We present a template-free particle-fusion approach based on an all-to-all registration that provides robustness against individual misregistrations and underlabeling. We achieved 3.3-nm Fourier ring correlation (FRC) image resolution by fusing 383 DNA origami nanostructures with 80% labeling density, and 5.0-nm resolution for structures with 30% labeling density.
UR - http://www.scopus.com/inward/record.url?scp=85053658858&partnerID=8YFLogxK
UR - http://resolver.tudelft.nl/uuid:0b2964e1-39af-4df9-8cd4-42e4ad7ddfd5
U2 - 10.1038/s41592-018-0136-6
DO - 10.1038/s41592-018-0136-6
M3 - Article
AN - SCOPUS:85053658858
SN - 1548-7091
VL - 15
SP - 781
EP - 784
JO - Nature Methods
JF - Nature Methods
ER -