Template-free 2D particle fusion in localization microscopy

Hamidreza Heydarian, Florian Schueder, Maximilian T. Strauss, Ben van Werkhoven, Mohamadreza Fazel, Keith A. Lidke, Ralf Jungmann, Sjoerd Stallinga, Bernd Rieger

Research output: Contribution to journalArticleScientificpeer-review

15 Citations (Scopus)
72 Downloads (Pure)

Abstract

Methods that fuse multiple localization microscopy images of a single structure can improve signal-to-noise ratio and resolution, but they generally suffer from template bias or sensitivity to registration errors. We present a template-free particle-fusion approach based on an all-to-all registration that provides robustness against individual misregistrations and underlabeling. We achieved 3.3-nm Fourier ring correlation (FRC) image resolution by fusing 383 DNA origami nanostructures with 80% labeling density, and 5.0-nm resolution for structures with 30% labeling density.

Original languageEnglish
Pages (from-to)781-784
JournalNature Methods
Volume15
DOIs
Publication statusPublished - 2018

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