The emerging landscape of single-molecule protein sequencing technologies

Peggy Bohländer; Mike Filius; Xander F. van Kooten; Adam Pomorski; Sonja Schmid; Cees Dekker; Rienk Eelkema; Sung Hyun Kim; Chirlmin Joo; null More Authors

doi:10.1038/s41592-021-01143-1

The emerging landscape of single-molecule protein sequencing technologies

Peggy Bohländer, Mike Filius, Xander F. van Kooten, Adam Pomorski, Sonja Schmid, Cees Dekker, Rienk Eelkema, Sung Hyun Kim, Chirlmin Joo, More Authors

Research output: Contribution to journal › Review article › peer-review

173 Citations (Scopus)

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Abstract

Single-cell profiling methods have had a profound impact on the understanding of cellular heterogeneity. While genomes and transcriptomes can be explored at the single-cell level, single-cell profiling of proteomes is not yet established. Here we describe new single-molecule protein sequencing and identification technologies alongside innovations in mass spectrometry that will eventually enable broad sequence coverage in single-cell profiling. These technologies will in turn facilitate biological discovery and open new avenues for ultrasensitive disease diagnostics.

Original language	English
Pages (from-to)	604-617
Number of pages	14
Journal	Nature Methods
Volume	18
Issue number	6
DOIs	https://doi.org/10.1038/s41592-021-01143-1
Publication status	Published - 2021

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10.1038/s41592-021-01143-1

Manuscript_03_10_2021_2015_Ref_TU3Accepted author manuscript, 1.57 MB

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AU - Schmid, Sonja

AU - Dekker, Cees

AU - Eelkema, Rienk

AU - Kim, Sung Hyun

AU - Joo, Chirlmin

AU - More Authors, null

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AB - Single-cell profiling methods have had a profound impact on the understanding of cellular heterogeneity. While genomes and transcriptomes can be explored at the single-cell level, single-cell profiling of proteomes is not yet established. Here we describe new single-molecule protein sequencing and identification technologies alongside innovations in mass spectrometry that will eventually enable broad sequence coverage in single-cell profiling. These technologies will in turn facilitate biological discovery and open new avenues for ultrasensitive disease diagnostics.

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JO - Nature Methods

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The emerging landscape of single-molecule protein sequencing technologies

Abstract

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