TY - JOUR
T1 - The profound influence of lipid composition on the catalysis of the drug target nadh type ii oxidoreductase
AU - Godoy-Hernandez, Albert
AU - McMillan, Duncan G.G.
PY - 2021
Y1 - 2021
N2 - Lipids play a pivotal role in cellular respiration, providing the natural environment in which an oxidoreductase interacts with the quinone pool. To date, it is generally accepted that negatively charged lipids play a major role in the activity of quinone oxidoreductases. By changing lipid compositions when assaying a type II NADH:quinone oxidoreductase, we demonstrate that phosphatidylethanolamine has an essential role in substrate binding and catalysis. We also reveal the importance of acyl chain composition, specifically c14:0, on membrane-bound quinone-mediated catalysis. This demonstrates that oxidoreductase lipid specificity is more diverse than originally thought and that the lipid environment plays an important role in the physiological catalysis of membrane-bound oxidoreductases.
AB - Lipids play a pivotal role in cellular respiration, providing the natural environment in which an oxidoreductase interacts with the quinone pool. To date, it is generally accepted that negatively charged lipids play a major role in the activity of quinone oxidoreductases. By changing lipid compositions when assaying a type II NADH:quinone oxidoreductase, we demonstrate that phosphatidylethanolamine has an essential role in substrate binding and catalysis. We also reveal the importance of acyl chain composition, specifically c14:0, on membrane-bound quinone-mediated catalysis. This demonstrates that oxidoreductase lipid specificity is more diverse than originally thought and that the lipid environment plays an important role in the physiological catalysis of membrane-bound oxidoreductases.
KW - Acyl chain
KW - Biomimetic membrane
KW - Lipids
KW - Type II NADH:quinone oxidoreductase
UR - http://www.scopus.com/inward/record.url?scp=85106892330&partnerID=8YFLogxK
U2 - 10.3390/membranes11050363
DO - 10.3390/membranes11050363
M3 - Article
AN - SCOPUS:85106892330
SN - 2077-0375
VL - 11
JO - Membranes
JF - Membranes
IS - 5
M1 - 363
ER -