The transcriptional regulator c2h2 acceleratesmushroom formation in Agaricus bisporus

Jordi F. Pelkmans, Aurin Vos, Karin Scholtmeijer, Ed Hendrix, Johan J.P. Baars, Thies Gehrmann, Marcel Reinders, Luis G. Lugones, Han A.B. Wösten

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The Cys2His2 zinc finger protein gene c2h2 of Schizophyllum commune is involved in mushroom formation. Its inactivation results in a strain that is arrested at the stage of aggregate formation. In this study, the c2h2 orthologue of Agaricus bisporus was over-expressed in this white button mushroom forming basidiomycete using Agrobacterium-mediated transformation. Morphology, cap expansion rate, and total number and biomass of mushrooms were not affected by over-expression of c2h2. However, yield per day of the c2h2 over-expression strains peaked 1 day earlier. These data and expression analysis indicate that C2H2 impacts timing of mushroom formation at an early stage of development, making its encoding gene a target for breeding of commercial mushroom strains.
Original languageEnglish
Pages (from-to)7151-7159
Number of pages9
JournalApplied Microbiology and Biotechnology
Issue number16
Publication statusPublished - 2016


  • Fungi
  • Basidiomycete
  • Agaricus bisporus
  • Mushroom
  • Transcription factor
  • Cys2His2

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