The transcriptional regulator c2h2 acceleratesmushroom formation in Agaricus bisporus

Jordi F. Pelkmans; Aurin Vos; Karin Scholtmeijer; Ed Hendrix; Johan J.P. Baars; Thies Gehrmann; Marcel Reinders; Luis G. Lugones; Han A.B. Wösten

doi:10.1007/s00253-016-7574-9

The transcriptional regulator c2h2 acceleratesmushroom formation in Agaricus bisporus

Jordi F. Pelkmans, Aurin Vos, Karin Scholtmeijer, Ed Hendrix, Johan J.P. Baars, Thies Gehrmann, Marcel Reinders, Luis G. Lugones, Han A.B. Wösten

Pattern Recognition and Bioinformatics

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Abstract

The Cys2His2 zinc finger protein gene c2h2 of Schizophyllum commune is involved in mushroom formation. Its inactivation results in a strain that is arrested at the stage of aggregate formation. In this study, the c2h2 orthologue of Agaricus bisporus was over-expressed in this white button mushroom forming basidiomycete using Agrobacterium-mediated transformation. Morphology, cap expansion rate, and total number and biomass of mushrooms were not affected by over-expression of c2h2. However, yield per day of the c2h2 over-expression strains peaked 1 day earlier. These data and expression analysis indicate that C2H2 impacts timing of mushroom formation at an early stage of development, making its encoding gene a target for breeding of commercial mushroom strains.

Original language	English
Pages (from-to)	7151-7159
Number of pages	9
Journal	Applied Microbiology and Biotechnology
Volume	100
Issue number	16
DOIs	https://doi.org/10.1007/s00253-016-7574-9
Publication status	Published - 2016

Keywords

Fungi
Basidiomycete
Agaricus bisporus
Mushroom
Transcription factor
Cys2His2

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10.1007/s00253-016-7574-9

11577347Final published version, 625 KBLicence: CC BY

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title = "The transcriptional regulator c2h2 acceleratesmushroom formation in Agaricus bisporus",

abstract = "The Cys2His2 zinc finger protein gene c2h2 of Schizophyllum commune is involved in mushroom formation. Its inactivation results in a strain that is arrested at the stage of aggregate formation. In this study, the c2h2 orthologue of Agaricus bisporus was over-expressed in this white button mushroom forming basidiomycete using Agrobacterium-mediated transformation. Morphology, cap expansion rate, and total number and biomass of mushrooms were not affected by over-expression of c2h2. However, yield per day of the c2h2 over-expression strains peaked 1 day earlier. These data and expression analysis indicate that C2H2 impacts timing of mushroom formation at an early stage of development, making its encoding gene a target for breeding of commercial mushroom strains.",

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AU - Pelkmans, Jordi F.

AU - Vos, Aurin

AU - Scholtmeijer, Karin

AU - Hendrix, Ed

AU - Baars, Johan J.P.

AU - Gehrmann, Thies

AU - Reinders, Marcel

AU - Lugones, Luis G.

AU - Wösten, Han A.B.

PY - 2016

Y1 - 2016

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AB - The Cys2His2 zinc finger protein gene c2h2 of Schizophyllum commune is involved in mushroom formation. Its inactivation results in a strain that is arrested at the stage of aggregate formation. In this study, the c2h2 orthologue of Agaricus bisporus was over-expressed in this white button mushroom forming basidiomycete using Agrobacterium-mediated transformation. Morphology, cap expansion rate, and total number and biomass of mushrooms were not affected by over-expression of c2h2. However, yield per day of the c2h2 over-expression strains peaked 1 day earlier. These data and expression analysis indicate that C2H2 impacts timing of mushroom formation at an early stage of development, making its encoding gene a target for breeding of commercial mushroom strains.

KW - Fungi

KW - Basidiomycete

KW - Agaricus bisporus

KW - Mushroom

KW - Transcription factor

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M3 - Article

SN - 0175-7598

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EP - 7159

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

IS - 16

ER -

The transcriptional regulator c2h2 acceleratesmushroom formation in Agaricus bisporus

Abstract

Keywords

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