Three demensional averaging for structural imaging of macromolecular complexes by localization microscopy

Hamidreza Heydarian, Adrian Przybylski, Florian Schreuder, Ralf Jungmann, Ben Van Werkhoven, Jan Keller-Findeisen, Jonas Ries, Sjoerd Stallinga, Mark Bates, Bernd Rieger

Research output: Contribution to journalArticleScientific

Abstract

Single molecule localization microscopy offers in principle resolution down to the molecularlevel, but in practice this is limited primarily by incompletefluorescent labeling of thestructure. This missing information can be completed by merging information from manystructurally identical particles. In this work, we present an approach for 3D single particleanalysis in localization microscopy which hugely increases signal-to-noise ratio and resolu-tion and enables determining the symmetry groups of macromolecular complexes. Ourmethod does not require a structural template, and handles anisotropic localization uncer-tainties. We demonstrate 3D reconstructions of DNA-origami tetrahedrons, Nup96 andNup107 subcomplexes of the nuclear pore complex acquired using multiple single moleculelocalization microscopy techniques, with their structural symmetry deducted from the data.
Original languageEnglish
Number of pages9
JournalbioRxiv
Publication statusE-pub ahead of print - 2021

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