Single molecule localization microscopy offers in principle resolution down to the molecularlevel, but in practice this is limited primarily by incompletefluorescent labeling of thestructure. This missing information can be completed by merging information from manystructurally identical particles. In this work, we present an approach for 3D single particleanalysis in localization microscopy which hugely increases signal-to-noise ratio and resolu-tion and enables determining the symmetry groups of macromolecular complexes. Ourmethod does not require a structural template, and handles anisotropic localization uncer-tainties. We demonstrate 3D reconstructions of DNA-origami tetrahedrons, Nup96 andNup107 subcomplexes of the nuclear pore complex acquired using multiple single moleculelocalization microscopy techniques, with their structural symmetry deducted from the data.
|Number of pages||9|
|Publication status||E-pub ahead of print - 2021|