Three dimensional particle averaging for structural imaging of macromolecular complexes by localization microscopy

Hamidreza Heydarian, Adrian Przybylski, Florian Schreuder, Ralf Jungmann, Ben Van Werkhoven, Jan Keller-Findeisen, Jonas Ries, Sjoerd Stallinga, Mark Bates, Bernd Rieger

Research output: Contribution to journalArticleScientific

Abstract

We present an approach for 3D particle fusion in localization microscopy which dramatically increases signal-to-noise ratio and resolution in single particle analysis. Our method does not require a structural template, and properly handles anisotropic localization uncertainties. We demonstrate 3D particle reconstructions of the Nup107 subcomplex of the nuclear pore complex (NPC), cross-validated using multiple localization microscopy techniques, as well as two-color 3D reconstructions of the NPC, and reconstructions of DNA-origami tetrahedrons.
Original languageEnglish
JournalbioRxiv
DOIs
Publication statusE-pub ahead of print - 2020

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  • Research Output

    • 1 Dissertation (TU Delft)

    Particle fusion in localization microscopy

    Heydarian, H., 2020, 96 p.

    Research output: ThesisDissertation (TU Delft)

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