Research Output per year
We present an approach for 3D particle fusion in localization microscopy which dramatically increases signal-to-noise ratio and resolution in single particle analysis. Our method does not require a structural template, and properly handles anisotropic localization uncertainties. We demonstrate 3D particle reconstructions of the Nup107 subcomplex of the nuclear pore complex (NPC), cross-validated using multiple localization microscopy techniques, as well as two-color 3D reconstructions of the NPC, and reconstructions of DNA-origami tetrahedrons.
Heydarian, H., Przybylski, A., Schreuder, F., Jungmann, R., Van Werkhoven, B., Keller-Findeisen, J., Ries, J., Stallinga, S., Bates, M., & Rieger, B. (2020). Three dimensional particle averaging for structural imaging of macromolecular complexes by localization microscopy. bioRxiv. https://doi.org/10.1101/837575