TY - JOUR
T1 - Towards a synthetic cell cycle
AU - Olivi, Lorenzo
AU - Berger, Mareike
AU - Creyghton, Ramon N.P.
AU - De Franceschi, Nicola
AU - Dekker, Cees
AU - Mulder, Bela M.
AU - Claassens, Nico J.
AU - ten Wolde, Pieter Rein
AU - van der Oost, John
PY - 2021
Y1 - 2021
N2 - Recent developments in synthetic biology may bring the bottom-up generation of a synthetic cell within reach. A key feature of a living synthetic cell is a functional cell cycle, in which DNA replication and segregation as well as cell growth and division are well integrated. Here, we describe different approaches to recreate these processes in a synthetic cell, based on natural systems and/or synthetic alternatives. Although some individual machineries have recently been established, their integration and control in a synthetic cell cycle remain to be addressed. In this Perspective, we discuss potential paths towards an integrated synthetic cell cycle.
AB - Recent developments in synthetic biology may bring the bottom-up generation of a synthetic cell within reach. A key feature of a living synthetic cell is a functional cell cycle, in which DNA replication and segregation as well as cell growth and division are well integrated. Here, we describe different approaches to recreate these processes in a synthetic cell, based on natural systems and/or synthetic alternatives. Although some individual machineries have recently been established, their integration and control in a synthetic cell cycle remain to be addressed. In this Perspective, we discuss potential paths towards an integrated synthetic cell cycle.
UR - http://www.scopus.com/inward/record.url?scp=85111763899&partnerID=8YFLogxK
U2 - 10.1038/s41467-021-24772-8
DO - 10.1038/s41467-021-24772-8
M3 - Review article
AN - SCOPUS:85111763899
SN - 2041-1723
VL - 12
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 4531
ER -