Towards electroenzymatic processes involving old yellow enzymes and mediated cofactor regeneration

Andreas Tosstorff, Cora Kroner, Diederik J. Opperman, Frank Hollmann, Dirk Holtmann

Research output: Contribution to journalArticleScientificpeer-review

10 Citations (Scopus)
16 Downloads (Pure)

Abstract

Old yellow enzymes are able to catalyze asymmetric C=C reductions. A mediated electroenzymatic process to regenerate the NADPH in combination with an old yellow enzyme was investigated. Due to the fact that the overall process was affected by a broad set of parameters, a design of experiments (DoE) approach was chosen to identify suitable process conditions. Process conditions with high productivities of up to 2.27 mM/h in combination with approximately 90% electron transfer efficiency were identified.

Original languageEnglish
Pages (from-to)71-76
Number of pages6
JournalEngineering in Life Sciences
Volume17
Issue number1
DOIs
Publication statusPublished - 2017

Keywords

  • C=C reduction
  • Design of experiments
  • Electroenzymatic synthesis
  • Mediated electron transfer
  • Old yellow enzyme

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