TY - JOUR
T1 - Towards electroenzymatic processes involving old yellow enzymes and mediated cofactor regeneration
AU - Tosstorff, Andreas
AU - Kroner, Cora
AU - Opperman, Diederik J.
AU - Hollmann, Frank
AU - Holtmann, Dirk
PY - 2017
Y1 - 2017
N2 - Old yellow enzymes are able to catalyze asymmetric C=C reductions. A mediated electroenzymatic process to regenerate the NADPH in combination with an old yellow enzyme was investigated. Due to the fact that the overall process was affected by a broad set of parameters, a design of experiments (DoE) approach was chosen to identify suitable process conditions. Process conditions with high productivities of up to 2.27 mM/h in combination with approximately 90% electron transfer efficiency were identified.
AB - Old yellow enzymes are able to catalyze asymmetric C=C reductions. A mediated electroenzymatic process to regenerate the NADPH in combination with an old yellow enzyme was investigated. Due to the fact that the overall process was affected by a broad set of parameters, a design of experiments (DoE) approach was chosen to identify suitable process conditions. Process conditions with high productivities of up to 2.27 mM/h in combination with approximately 90% electron transfer efficiency were identified.
KW - C=C reduction
KW - Design of experiments
KW - Electroenzymatic synthesis
KW - Mediated electron transfer
KW - Old yellow enzyme
UR - http://resolver.tudelft.nl/uuid:bbe2acac-2a1c-42f8-b667-abda872538f2
UR - http://www.scopus.com/inward/record.url?scp=84991051791&partnerID=8YFLogxK
U2 - 10.1002/elsc.201600158
DO - 10.1002/elsc.201600158
M3 - Article
AN - SCOPUS:84991051791
SN - 1618-0240
VL - 17
SP - 71
EP - 76
JO - Engineering in Life Sciences
JF - Engineering in Life Sciences
IS - 1
ER -