Towards electroenzymatic processes involving old yellow enzymes and mediated cofactor regeneration

Andreas Tosstorff; Cora Kroner; Diederik J. Opperman; Frank Hollmann; Dirk Holtmann

doi:10.1002/elsc.201600158

Towards electroenzymatic processes involving old yellow enzymes and mediated cofactor regeneration

Andreas Tosstorff, Cora Kroner, Diederik J. Opperman, Frank Hollmann, Dirk Holtmann^*

^*Corresponding author for this work

BT/Biocatalysis

Research output: Contribution to journal › Article › Scientific › peer-review

12 Citations (Scopus)

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Abstract

Old yellow enzymes are able to catalyze asymmetric C=C reductions. A mediated electroenzymatic process to regenerate the NADPH in combination with an old yellow enzyme was investigated. Due to the fact that the overall process was affected by a broad set of parameters, a design of experiments (DoE) approach was chosen to identify suitable process conditions. Process conditions with high productivities of up to 2.27 mM/h in combination with approximately 90% electron transfer efficiency were identified.

Original language	English
Pages (from-to)	71-76
Number of pages	6
Journal	Engineering in Life Sciences
Volume	17
Issue number	1
DOIs	https://doi.org/10.1002/elsc.201600158
Publication status	Published - 2017

Keywords

C=C reduction
Design of experiments
Electroenzymatic synthesis
Mediated electron transfer
Old yellow enzyme

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10.1002/elsc.201600158

OYE and DoE Engineering in Life Science_revisedAccepted author manuscript, 848 KB

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AU - Kroner, Cora

AU - Opperman, Diederik J.

AU - Hollmann, Frank

AU - Holtmann, Dirk

PY - 2017

Y1 - 2017

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AB - Old yellow enzymes are able to catalyze asymmetric C=C reductions. A mediated electroenzymatic process to regenerate the NADPH in combination with an old yellow enzyme was investigated. Due to the fact that the overall process was affected by a broad set of parameters, a design of experiments (DoE) approach was chosen to identify suitable process conditions. Process conditions with high productivities of up to 2.27 mM/h in combination with approximately 90% electron transfer efficiency were identified.

KW - C=C reduction

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KW - Electroenzymatic synthesis

KW - Mediated electron transfer

KW - Old yellow enzyme

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Towards electroenzymatic processes involving old yellow enzymes and mediated cofactor regeneration

Abstract

Keywords

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