TY - JOUR
T1 - Towards enhanced n-butanol production from sugarcane bagasse hemicellulosic hydrolysate
T2 - Strain screening, and the effects of sugar concentration and butanol tolerance
AU - Zetty-Arenas, Ana Maria
AU - Alves, Rafael Ferraz
AU - Portela, Carla Andreia Freixo
AU - Mariano, Adriano Pinto
AU - Basso, Thiago Olitta
AU - Tovar, Laura Plazas
AU - Maciel Filho, Rubens
AU - Freitas, Sindelia
PY - 2019
Y1 - 2019
N2 - Robust strains are essential towards success of n-butanol production from lignocellulosic feedstock. To find a suitable strain to convert a non-detoxified hemicellulosic hydrolysate of sugarcane bagasse, we first assessed the performance of four wild-type butanol-producing Clostridium strains (C. acetobutylicum DSM 6228, C. beijerinckii DSM 6422, C. saccharobutylicum DSM 13864, and C. saccharoperbutylacetonicum DSM 14923)in batch fermentations containing either xylose or glucose at 30 g L−1 as sole carbon sources. C. saccharoperbutylacetonicum was selected after achieving butanol yields as high as 0.31 g g−1 on glucose and 0.25 g g−1 on xylose. In a 48-h fermentation containing a mixture of sugars (93% xylose and 7% glucose)that mimicked the hydrolysate, C. saccharoperbutylacetonicum delivered the highest butanol concentration (14.5 g L−1)when the initial sugar concentration was 50 g L−1. Moreover, the selected strain achieved the highest butanol yield (0.29 g g−1)on xylose-rich media reported so far. Meanwhile, C. saccharoperbutylacetonicum produced 5.8 g butanol L−1 (0.22 g g−1 butanol yield)when fermenting a non-detoxified sugarcane bagasse hemicellulosic hydrolysate enriched with xylose (30 g total sugars L−1). Although sugars were not exhausted (4.7 g residual sugars L−1)even after 72 h because of the presence of lignocellulose-derived microbial inhibitors, these results show that C. saccharoperbutylacetonicum is a robust wild-type strain. This microorganism with high butanol tolerance and yield on xylose can, therefore, serve as the basis for the development of improved biocatalysts for production of butanol from non-detoxified sugarcane bagasse hemicellulosic hydrolysate.
AB - Robust strains are essential towards success of n-butanol production from lignocellulosic feedstock. To find a suitable strain to convert a non-detoxified hemicellulosic hydrolysate of sugarcane bagasse, we first assessed the performance of four wild-type butanol-producing Clostridium strains (C. acetobutylicum DSM 6228, C. beijerinckii DSM 6422, C. saccharobutylicum DSM 13864, and C. saccharoperbutylacetonicum DSM 14923)in batch fermentations containing either xylose or glucose at 30 g L−1 as sole carbon sources. C. saccharoperbutylacetonicum was selected after achieving butanol yields as high as 0.31 g g−1 on glucose and 0.25 g g−1 on xylose. In a 48-h fermentation containing a mixture of sugars (93% xylose and 7% glucose)that mimicked the hydrolysate, C. saccharoperbutylacetonicum delivered the highest butanol concentration (14.5 g L−1)when the initial sugar concentration was 50 g L−1. Moreover, the selected strain achieved the highest butanol yield (0.29 g g−1)on xylose-rich media reported so far. Meanwhile, C. saccharoperbutylacetonicum produced 5.8 g butanol L−1 (0.22 g g−1 butanol yield)when fermenting a non-detoxified sugarcane bagasse hemicellulosic hydrolysate enriched with xylose (30 g total sugars L−1). Although sugars were not exhausted (4.7 g residual sugars L−1)even after 72 h because of the presence of lignocellulose-derived microbial inhibitors, these results show that C. saccharoperbutylacetonicum is a robust wild-type strain. This microorganism with high butanol tolerance and yield on xylose can, therefore, serve as the basis for the development of improved biocatalysts for production of butanol from non-detoxified sugarcane bagasse hemicellulosic hydrolysate.
KW - Butanol tolerance
KW - Clostridium saccharoperbutylacetonicum
KW - Hemicellulosic hydrolysate
KW - Wild-type strain
KW - Xylose
UR - http://www.scopus.com/inward/record.url?scp=85066235783&partnerID=8YFLogxK
U2 - 10.1016/j.biombioe.2019.05.011
DO - 10.1016/j.biombioe.2019.05.011
M3 - Article
AN - SCOPUS:85066235783
SN - 0961-9534
VL - 126
SP - 190
EP - 198
JO - Biomass and Bioenergy
JF - Biomass and Bioenergy
ER -