Towards enhanced n-butanol production from sugarcane bagasse hemicellulosic hydrolysate: Strain screening, and the effects of sugar concentration and butanol tolerance

Ana Maria Zetty-Arenas, Rafael Ferraz Alves, Carla Andreia Freixo Portela, Adriano Pinto Mariano, Thiago Olitta Basso, Laura Plazas Tovar, Rubens Maciel Filho, Sindelia Freitas

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9 Citations (Scopus)

Abstract

Robust strains are essential towards success of n-butanol production from lignocellulosic feedstock. To find a suitable strain to convert a non-detoxified hemicellulosic hydrolysate of sugarcane bagasse, we first assessed the performance of four wild-type butanol-producing Clostridium strains (C. acetobutylicum DSM 6228, C. beijerinckii DSM 6422, C. saccharobutylicum DSM 13864, and C. saccharoperbutylacetonicum DSM 14923)in batch fermentations containing either xylose or glucose at 30 g L−1 as sole carbon sources. C. saccharoperbutylacetonicum was selected after achieving butanol yields as high as 0.31 g g−1 on glucose and 0.25 g g−1 on xylose. In a 48-h fermentation containing a mixture of sugars (93% xylose and 7% glucose)that mimicked the hydrolysate, C. saccharoperbutylacetonicum delivered the highest butanol concentration (14.5 g L−1)when the initial sugar concentration was 50 g L−1. Moreover, the selected strain achieved the highest butanol yield (0.29 g g−1)on xylose-rich media reported so far. Meanwhile, C. saccharoperbutylacetonicum produced 5.8 g butanol L−1 (0.22 g g−1 butanol yield)when fermenting a non-detoxified sugarcane bagasse hemicellulosic hydrolysate enriched with xylose (30 g total sugars L−1). Although sugars were not exhausted (4.7 g residual sugars L−1)even after 72 h because of the presence of lignocellulose-derived microbial inhibitors, these results show that C. saccharoperbutylacetonicum is a robust wild-type strain. This microorganism with high butanol tolerance and yield on xylose can, therefore, serve as the basis for the development of improved biocatalysts for production of butanol from non-detoxified sugarcane bagasse hemicellulosic hydrolysate.

Original languageEnglish
Pages (from-to)190-198
JournalBiomass and Bioenergy
Volume126
DOIs
Publication statusPublished - 2019

Keywords

  • Butanol tolerance
  • Clostridium saccharoperbutylacetonicum
  • Hemicellulosic hydrolysate
  • Wild-type strain
  • Xylose

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    Zetty-Arenas, A. M., Alves, R. F., Portela, C. A. F., Mariano, A. P., Basso, T. O., Tovar, L. P., Maciel Filho, R., & Freitas, S. (2019). Towards enhanced n-butanol production from sugarcane bagasse hemicellulosic hydrolysate: Strain screening, and the effects of sugar concentration and butanol tolerance. Biomass and Bioenergy, 126, 190-198. https://doi.org/10.1016/j.biombioe.2019.05.011